Of 53 strains of Neisseria gonorrhoeae isolated in Benin City, Nigeria, in February 1983 to October 1984, 46 (87%) produced penicillinase. The minimum inhibitory concentrations (MICs) of penicillin G and ampicillin for these isolates were between 1 mg/l and and 50 mg/l. About 48% (22/46) of the penicillinase producing strains were also resistant to streptomycin, cotrimoxazole, and ampicillin an...

The rapid fixed-time assay for penicillinase was modified for measuring beta-lactamase activity with twelve substrates, i.e., benzylpenicillin, ampicillin, cloxacillin, methicillin, carbenicillin, cefazolin, cephalothin, cephaloglycin, cephalexin, cephalosporin C, 7-aminocephalosporanic acid, and cefoxitin. The method depends upon the reduction of iodine by the hydrolyzed substrate. Determined ...

An inducible penicillin beta-lactamase was purified from a strain of Alcaligenes faecalis resistant to beta-lactam antibiotics. The purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis, and its molecular weight was 29,000 based on sodium dodecyl sulfate-acrylamide gel electrophoresis. Its isoelectric point was 5.9. The enzyme more rapidly hydrolyzed penic...

Wide range of biological activity of isoxazole makes it an important anchor in the field of medicinal chemistry. Already marketed drugs like Valdecoxib as COX-2 inhibitor, Cloxacillin and Dicloxacillin as beta lactamase resistant antibiotics having isoxazole core has beenproven to be efficacious drugs in present respective disease scenario. Therefore synthesis and evaluation of isoxazole contai...

The World Health Organization has produced guidelines for the management of common illnesses in hospitals with limited resources. This series reviews the scientific evidence behind WHO’s recommendations. The WHO guidelines, and more reviews are available at http://www.who.int/child-adolescenthealth/publications/CHILD_HEALTH/PB.htm. This review addresses the question: ‘‘What are the most appropr...

Abstract Objectives To investigate if the addition of cloxacillin to vancomycin enhances activity both monotherapies for treating MSSA and MRSA experimental endocarditis (EE) in rabbits. Methods Vancomycin plus was compared with respective daptomycin. In vitro time–kill studies were performed using standard (105 cfu) high (108 inocula five MRSA, one glycopeptide-intermediate (GISA) strains. One...

Due to slow diagnostics, physicians must optimize antibiotic therapies based on clinical evaluation of patients without specific information on causative bacteria. We have investigated metabolomic analysis of blood for the detection of acute bacterial infection and early differentiation between ineffective and effective antibiotic treatment. A vital and timely therapeutic difficulty was thereby...