Mastitis is considered one of the most important diseases of domestic animals, caused by several etiologic agents. Transmission of the microorganisms primarily occurs by ascendant via in canal of teats, usually involving agents from animals and environmental origin and from milking process (Anderson et al., 2005). Staphylococcus aureus is recognized as the most common causal agent of goat masti...

Nine Clostridium perfringens strains were isolated from fifteen intestinal content samples collected from commercial broilers which suffered from necrotic enteritis NE. Molecular typing of C. perfringens was performed by PCR using three sets of primers specific for toxin -producing genes of C. perfringens [alpha (900bp), beta (236bp) and epsilon (541bp)]. Six strains of C. perfringens type A an...

Evidence has accumulated indicating that the clostridia ferment glucose by means of a system similar to the Embden-Meyerhof-Parnas (EMP) pathway (Elsden, 1952). In the case of Clostridium perfringens, for instance, most of the enzymes of the EMP pathway have been demonstrated in cell free extracts (Bard and Gunsalus, 1950; Shankar and Bard, 1955). Further evidence for the operation of the EMP p...

Clostridium perfringens is a major cause of histotoxic and intestinal infections of humans and other animals. This Gram-positive anaerobic bacterium can produce up to three sialidases named NanH, NanI, and NanJ. The role of sialidases in histotoxic infections, such as gas gangrene (clostridial myonecrosis), remains equivocal. However, recent in vitro studies suggest that NanI may contribute to ...

The genus Clostridium is a Gram-positive, spore-forming bacterium. Most species of Clostridium spp. usually are found in soil, water, dead plants, animal carcasses, and play an important role the decomposition of substances nature. Raw meat fresh vegetable more likely to be contaminated with spores or vegetative cells genus Clostridium. study results showed t...

Published criteria for implicating Clostridium perfringens as the cause of food-poisoning outbreaks include finding a median fecal C. perfringens spore count of greater than 10(6)/g among specimens from ill persons. We investigated a food-poisoning outbreak with the epidemiologic characteristics of C. perfringens-related disease in a nursing home in which the median fecal spore count for ill pa...

Sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative estimation of Clostridium perfringens enterotoxin (CPE) with monoclonal and polyclonal antibodies as capturing and detecting antibodies, respectively. The dose-dependent relationship between absorbance at 405 nm and concentration of purified CPE was obtained over the range of 0.64-400 ng/ml. The sandwich ELISA...

A new apparatus was developed for measuring changes in E(h), pH, and cell numbers. With this apparatus, the relationships of these parameters were studied at initial E(h) levels of 200 and 40 mv (pH 7.0), by using Clostridium perfringens and Pseudomonas fluorescens. One of the strains of C. perfringens grew more luxuriantly at the higher E(h), in the presence of small quantities of oxygen, than...

Oligonucleotide probes designed on the basis of the N-terminal sequence of Clostridium perfringens beta-toxin were used to isolate the encoding gene (cpb). The nucleotide sequence of cpb was determined, and on the basis of DNA hybridization experiments it was shown that the gene is found only in type B and C strains of C. perfringens. The deduced amino acid sequence of the beta-toxin revealed h...

The Gram-positive anaerobic bacterium Clostridium perfringens is widely distributed in nature, especially in soil and the gastrointestinal tracts of humans and animals. C. perfringens causes gas gangrene and food poisoning, and it produces extracellular enzymes and toxins that are thought to act synergistically and contribute to its pathogenesis. A complicated regulatory network of toxin genes ...