In view of the bad convergence performance and low precision of standard artificial fish swarm algorithm in the water quality properties identification, this paper put forward an improved identification model based on adaptive parameters optimization. Firstly, it optimized the immune cloning and selection algorithm (ICSA) in periodic mutation operator and selection operator. Then it introduced ...

New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...

Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study, a novel strategy based on phage ϕBT1 integrase-mediated site-specific recombination was developed, and used for simultaneous Streptomyces gen...

Genetic algorithms are commonly used to perform searches on complex search spaces for optimum solutions of many realworld problems. The evolvable hardware domain presents many problems with complex search spaces subject to the use of a genetic algorithm as an optimization technique for field programmable gate arrays (FPGA) implementations. The optimization of FPGA implementations, in general, a...

With regard to human cloning or artificial human reproduction – and contrary to the opinions of Sunni scholars - Shiite thinkers have not held a unified position. After having surveyed a number of Shiite fatwas and analyses on the subject, this essay will classify them into four groups. The first group states that we are granted absolute permission to engage in human cloning; while the second g...

In the present study, we experimentally tested the role of hand motor circuits in simple-arithmetic strategies. Educated adults solved simple additions (e.g., 8 + 3) or simple subtractions (e.g., 11 - 3) while they were required to retrieve the answer from long-term memory (e.g., knowing that 8 + 3 = 11), to transform the problem by making an intermediate step (e.g., 8 + 3 = 8 + 2 + 1 = 10 + 1 ...

Many different strategies are used for cloning polymerase chain reaction (PCR) products. Some use restriction sites pre-integrated into primers or contained in a generated fragment. Others, such as the one used by Stratagene (La Jolla, CA, USA) in its pCR-Script Direct SK(+) Cloning Kit, are based on a blunt-end ligation. These strategies require the use of additional enzymes to polish the end...

BACKGROUND Heterologous expression of biosynthetic gene clusters of natural microbial products has become an essential strategy for titer improvement and pathway engineering of various potentially-valuable natural products. A Streptomyces artificial chromosomal conjugation vector, pSBAC, was previously successfully applied for precise cloning and tandem integration of a large polyketide tautomy...

We have investigated the use of soluble chimeric trimers of the major capsid protein VP7 of African horse sickness virus (AHSV) as a vaccine delivery system by targeting some of the natural hydrophilic loops on the VP7 top domain for the insertion of foreign peptides. Key to this trimer display strategy is the solubility of AHSV VP7 and how the solubility of this hydrophobic protein can be mani...

The advent of New Zealand’s world-leading Digital Strategy underpins the increasing importance of ICTs in community settings. It also marks a significant change in government policy and the first time a government has adopted a whole of government approach to ICTs. This article provides a personal view of the policy process for creating the Digital Strategy, presented by one of the participants...