REPORTS Recombinant DNA: Examples ofPresent-Day Research:J.Abelson. Charon Phages: Safer Derivatives ofBacteriophage Lambda forDNA Cloning: F. R. Blattner et al. . Recombinational Switch for Gene Expression: J. Zieg et al. Gene Cloning and Containment Properties of Plasmid Col El and Its Derivatives: K. A. Armstrong, V. Hershfield, D. R. Helinski. EK2 Derivatives ofBacteriophage Lambda Useful i...

Banning therapeutic cloning would block research that could lead to cures for many diseases. In therapeutic cloning, genetic material from an adult cell is placed inside an egg to grow beneficial stem cells, not to produce a baby. Therapeutic cloning would allow a patient's own genetic material to be used to repair his or her damaged cells. There is broad consensus in the scientific community t...

This paper examines the current Australian regulatory response to human reproductive cloning. The central consideration is the capacity of the current regulatory regime to effectively deter human cloning efforts. A legislative prohibition on human cloning must be both effective and clear enough to allow researchers to know what practices are acceptable. This paper asks whether the current Austr...

This paper revisits the universal asymmetric 1 → 2 quantum cloning problem. We identify the symmetry properties of this optimization problem, giving us access to the optimal quantum cloning map. Furthermore, we use the bipolar theorem, a famous method from convex analysis, to completely characterize the set of achievable single quantum clone qualities using the fidelity as our figure of merit; ...

Zebrafish genome sequencing project has improved efficiency of positional cloning in zebrafish and traditional chromosome walking by isolating large insert genomic libraries has become a past. However, the genetic principles underlying the positional cloning still form the foundation for current chromosome walking using the genome sequence assemblies and related genomic sequence and clone infor...

Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to m...

Research suggests that cloning is incapable of producing a viable embryo when it is used on primate eggs. In fact, the entity created may not qualify as an embryo at all. If the results stand, cloning avoids the moral objections typically lodged against it, and cloning is itself an "alternative source" of stem cells.

Dolly the sheep cloning “the first cloning process” was derived by somatic cell nuclear transfer and it had reported that the clone got premature senescence as a result of continues telomere shortening and ongoing decrease in telomerase activity by time; thus majority of recent researches is directed to use other types of donor cells to be transferred into the recipient enucleated oocyte to mai...

Code duplication is a well documented problem in industrial software systems. There has been considerable research into techniques for detecting duplication in software, and there are several effective tools to perform this task. However, there have been few detailed qualitative studies into how cloning actually manifests itself within software systems. This is primarily due to the large result...