To analyze gene regulatory networks active during embryonic development and organogenesis it is essential to precisely define how the different genes are expressed in spatial relation to each other in situ. Multi-target chromogenic whole-mount in situ hybridization (MC-WISH) greatly facilitates the instant comparison of gene expression patterns, as it allows distinctive visualization of differe...

BACKGROUND The potential problems associated with the use of formalin in histology, such as health hazards, degradation of RNA and cross-linking of proteins are well recognized. We describe the utilization of a formalin-free fixation and processing system for tissue detection of two important biopredictors in breast cancer - estrogen receptor and HER2 - at the RNA and protein levels. METHODS ...

In cases of benign lesions, pelviscopy is used in about 70% of all abdominal operations at our Department of Obstetrics and Gynecology. From 1990 to 1992, 851 patients with myomas were treated by surgery. In 57% pelviscopy, in 2% laparotomy, and in 1% hysteroscopic myomectomies were treated. In 11%, a CISH (Classical Intrafascial SEMM-serrated edged macro morcellator-Hysterectomy) without colpo...

Breast carcinoma is the most common malignant tumor and leading cause of cancer death in women, with more than 1,000,000 cases occurring worldwide annually (Rosai, 2004). According to the survey of epidemiology and end results (SEER) data, the world wide incidence of breast carcinoma is 123 women per 100,000 and its incidence in Asia is 89.5 per 100,000 (Horner et al., 2009). The breast carcino...