To examine the composition of lanostanoids in Ganoderma lucidum, we have developed a liquid chromatography-mass spectrometry (LC-MS) method by using the ganoderic acids isolated in our laboratory as reference standards. The identity of 14 peaks in the high performance liquid chromatogram (HPLC) of G. lucidum has been confirmed. By using the HPLC retention times of these ganoderic acids and thei...

inadvertently allowed to dry within or on the tip of the needle. We have been using chloroform as extracting solvent for over a year, but have not seen the problems encountered by Goudie et al. concerning detector contamination, sensitivity, and baseline noise. The baseline noise they referred to in our chromatogram is not related to the use of chloroform. The degree of noise is common to the o...

A description is given of a chemometric approach used to extract information on the characteristics of n-alkane and n-alkanoic acid homologous series as useful markers for PM source identification and differentiation. The key parameters of the homologous series – number of terms and Carbon Preference Index – are directly estimated by the Autocovariance Function (EACVF) computed on the acquired ...

An anion-exchange high-performance liquid chromatographic procedure was developed for the separation of transferrin in human plasma. The procedure allowed the four molecular forms of transferrin, which differ with respect to bound iron, to be separated from each other and from other plasma proteins. Transferrin variants, including B and D types, could also be identified using anion-exchange hig...

Triacylglycerol (TAG) composition in vegetable oils was collaboratively analyzed in 9 laboratories using high-performance liquid chromatography (HPLC) equipped with a refractive index detector. Dococyl (C22) and triacontyl (C30) silica columns were used for analysis. The TAG molecular species in soybean, rapeseed, and palm oils were individually separated on the chromatogram. The collaborative ...

The physicochemical properties, composition and antimicrobial activity of cinnamon essential oil (Cinnamomum zeylanicum) were studied. The bioactivity of this essential oil against Paenibacillus larvae was analyzed by means of a combination of in vitro techniques, such as the tube dilution method and bioautography, a method employed to localize antibacterial activity on a chromatogram. Cinnamal...

This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and...

Hydrocortisone (HC) inhibited the growth of HeLa cells at the concentration of 0.01•`10ƒÊg/ml but enhanced it at 0.001ƒÊg/ml. HC increased the ƒÀ-glucuronidase and alkaline phosphatase activities of HeLa cells at 10ƒÊg/ml in 4 days, although it did not affect the acid phosphatase activity. Nucleic acids obtained from growing HeLa cells were fractionated by methylated bovine serum albumin column...

The Agilent Technologies mass selective detector (MSD) coupled with deconvolution reporting software (DRS) provides additional powerful data processing capabilities to the MSD ChemStation software. Reviewing full scan gas chromatography/mass spectrometry data for the confirmation of pesticide residues can be a labor-intensive and time-consuming process requiring great skill and concentration by...

A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Pueraria tuberose was developed and described. The method involves separation of active compound using mobile phase consisting of acetonitrile: water: orthophosporic acid in the ratio of 32:67:01 and detecting the chromatogram at 280 nm using photodiode array detector. The sensitivity of the method was ob...