The sensitivity of the scrapie agent to u.v. inactivation was found to be related to the purity of the tissue preparation. Scrapie infectivity associated with membrane vesicles was unaffected when irradiated with 10(4) J/m2. Irradiation of more highly purified preparations from detergent-extracted CsCl gradient fractions reduced scrapie infectivity from 10(7.8) log10 LD50 per ml to as low as 10...

A method is described for estimating plasma and urinary hemoglobin concentrations as low as 3 mg/L. The assay measures at 528 nm the rate of peroxidation of chlorpromazine by hemoglobin and is not affected by ascorbate and bilirubin concentrations up to 500 mumol/L. Results by this method (mean +/- SD: 54.4 +/- 41.6 mg/L; n = 19) correlated well with those by a scanning spectrophotometric metho...

Acute renal papillary necrosis (RPN) in animals is characterized by increased renal lipid accumulation. The excretion of renal lipids into urine has been determined to evaluate their possible use as sensitive early biomarkers for the diagnosis of RPN. This study investigates injury induced by two model nephrotoxins, mefenamic acid (MFA), a non-steroidal anti-inflammatory drug (NSAID), and its a...

Interactions between herbicides and vesicular-arbuscular mycorrhizal (VAM) fungi are little-known but may differentially affect the development of tolerant and susceptible plants. We conducted this greenhouse study to determine if VAM fungi modify the effects of the herbicide bentazon (3isopropyllH-2 ,1 ,3-benzothid iaz in(4)3H-one 2,2-dioxide) on soybean (Glyc ine max (L.) Merr.) and common co...

CHEST 71: 2, FEBRUARY, 1977 SUPPLEMENT RVEF, cardiac index (CI), and mean pulmonary artery pressure (PAP) were measured before and 30 minutes after intravenous ouabain (0.01 mgkg) in 11 patients with severe CAO and DCP. Control RVEF (46.9 ± 3.4%), CI (2.79 0.15 L/min/M2) and PAP (30 ± 2 mm Hg) were not significantly altered by ouabain (48.5 ± 2.9%, 2.82 ± 0.14 L/min/M2, 31 ± 1 mm Hg respectivel...

During both acute and chronic mineralocorticoid administration, parotid saliva was obtained by acetylcholine stimulation at rates of 1-0—1 -5 mlmin" from anaesthetized red kangaroos. The Na/K ratio of saliva from chronically Na-replete kangaroos was virtually unaltered by ipsilateral intracarotid infusion of aldosterone at rates of 8, 40 or 80/igh" for 4h, the ratio falling from 20-1 ± 1-09 to ...