Mycelial and yeast-phase culture filtrates prepared from three strains of Paracoccidioides brasiliensis exhibited equal reactivity in sensitized guinea pigs. Ethyl alcohol-precipitated fractions obtained from the culture filtrates also showed no difference in reactivity between mycelial and yeast phase when tested in sensitized guinea pigs. Chemical analyses of the ethyl alcohol-precipitated fr...

The present study was designed to detect the class of compound by phytochemical evaluation and perform high performance thin layer chromatography (HPTLC) fingerprint analysis hexane extract P. quadrifida (HEPQ), chloroform (CEPQ), ethyl acetate (EAEPQ) ethanol (EEPQ). successive extraction based on eluotropic solvent system utilised for extraction. preliminary screening carried out all extract....

nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. the aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. the presence or absence of the methicillin-resistance gene in 115 clinical isolates of staphylococcus aureus and 50 isolates of coagulase negative staphy...

Tissues were made senescent by inhibiting synthetic reactions with abscisic acid and by accelerating protein catabolism with L-serine in combination with kinetin. Squash (Cucurbita maxima) leaf discs were floated on the above-mentioned solutions, harvested, and the lipids extracted therefrom. Fatty acids of the acyl lipids were determined by gas chromatography, galactolipid concentrations were ...

BACKGROUND Dengue is listed as a neglected tropical disease by the Center for Disease Control and Preservation, as there are insufficient integrated surveillance strategies, no effective treatment, and limited licensed vaccines. Consisting of four genetically distinct serotypes, dengue virus (DENV) causes serious life-threatening infections due to its complexity. Antibody-dependent enhancement ...

Abstract This study assessed the performance of five different DNA extraction methods for recovery from bone: ChargeSwitch® gDNA Plant Kit, IQ™ System DNeasy® Blood & Tissue PrepFiler® BTA Forensic Extraction Kit and phenol-chloroform-isoamyl alcohol. was extracted pig rib femur bones that fresh, had undergone surface decomposition three months, one year. Extracted analyzed using real-...

A simple reversed flow injection system with minimal consumption of organic solvent has been developed for the spectrophotometric determination of anionic surfactant (AS) in water samples. The method is based on the ion-pair formation between anionic surfactant and Methylene Blue (MB) and its extraction to chloroform. Sodium dodecyl sulfate (SDS) is used as a model anionic surfactant. The sampl...

Dispersive liquid-liquid microextraction (DLLME) combined with gas chromatography-mass spectrometry (GC-MS) was used for the extraction and determination of 13 polycyclic aromatic hydrocarbons (PAHs) in mineral water samples. In this procedure, the suitable combination of extraction solvent (500 µL chloroform) and disperser solvent (1000 µL acetone) were quickly injected into the water sample (...

modified bubble column extraction with ultrasonic bath (bce-ub) method was used to extract atropine from the stem and leaves of atropa belladonna. optimum condition were obtained with kamada solvent which was chloroform-methanol-ammonia 15:15:1(v/v/v) as extraction solvent, the particle size of  less than  350 µm, an extraction time of 23.95 min, a liquor to material ratio of 15.08 ml/g and  an...

an ultrasound-assisted surfactant-enhanced emulsification microextra- ction (uaseme) coupled with high performance liquid chromatography-diode array detection has been developed for the extraction and determination of thiopental in serum and urine samples. a simple microextraction method based on emulsification of organic extraction solvent in aqueous sample using an ionic surfactant and withou...