The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular...

Anomericformoftheproductsfremchitinase-catalyzedhydrolysis was analyzed by iH-NMR spectroscopy. When the chitinase from StreptenoTces griseus was added to the solution of substrate, Ai,AJ,IV,IV;IV-pentaacetylchitopentaitol, fi-anomer was first produced by the enzymatic hydrolysis, and then transformed to oc-anomer by mlltarotatioo. In contrast, the chitinase from Dioscorea qmpesita (yam) produc...

UNLABELLED A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalytic domain. This catalytic domain shows no similarity with previously characte...

An open reading frame encoding the chitinase gene and its signal sequence was cloned from the Vibrio parahaemolyticus KN1699 genome. An expression plasmid containing the gene was introduced into Escherichia coli cells, and recombinant chitinase (Pa-rChi) was produced and secreted into the culture medium with the aid of the signal peptide. Pa-rChi was purified and its substrate specificity was d...

A new chitinase inhibitor, named argifin, was isolated from the cultured broth of a fungal strain FTD-0668. The strain was identified as Gliocladium sp. from morphological characteristics. The IC50 value of argifin against Lucilia cuprina chitinase was 3.7 microM. Argifin arrested the moult of cockroach larvae upon injection into the ventral abdominal part.

Potato (Solanum tuberosum L.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including Rhizoctonia solani (AG-3), the causal agent of stem and root rot diseases. Chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. In the present study, plasmid p...

fusarium wilt caused by fusarium oxysporum f.sp. lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. in order to increase the tolerance to this disease, a triple structure containing pr1, chitinase and glucanase genes controlled by 35s promoter was transferred to tomato. eight days after planting on pre-culture medium, exp...