From chickpea (Cicer arietinum L.), one ,f3-1,3-glucanase and two chitinases were purified and characterized (Vogelsang and Barz, 1993). The P-l,S-glucanase and one chitinase each with a basic isoelectric point (class I) were shown to be localized in vacuoles, whereas the second class 111 acidic chitinase revealed an extracellular localization (Mackenbrock et al., 1992). Here we report on the c...

conclusions in conclusion, chit1 24 bp duplication might not be a candidate gene for susceptibility to ptb. larger studies are necessary to confirm these findings in various populations. results homozygous wild type, heterozygous and homozygous mutant frequencies of chit1 24 bp duplication polymorphism were 43.9%, 43.9% and 12.2% in controls and 42.8%, 45.1% and 12.1% in ptb patients. we found ...

A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...

A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various ...

Phytophthora-infected plants. These results suggest some diversity in the chitinase activities concerning New chitosanase acidic isoforms have been shown in substrate specificity in mycorrhizal plants. The Glomus mosseae-colonized tomato roots and their possible implications of these observations in the induction, together with the previously described functioning of the symbiosis is discussed....

The faecal material of seven species of attine ants from the genera Cyphomyrmex, Apterostigma, Myrmicocrypta, Sericomyrmex, and Atta has been shown to contain c+amylase and chitinase, but only a trace of uricase. Chitinase probably serves a beneficial rBle in the fungus-culturing activities of the primitive form.3 by contributing to the degradation of chitinous substrates, such as insect cuticl...

We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,O18 nucleotides and includes an open reading frame encoding a po]ypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase ac...

A chitinase was purified from Vitis vinifera Manzoni Bianco grape juice and characterized. On the basis of proteomic analysis of tryptic peptides, a significant match identified the enzyme as a type IV grape chitinase previously found in juices of other V. vinifera varieties. The optimal pH and temperature for activity toward colloidal chitin were found to be 6 and 30 °C, respectively. The enzy...

Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, Pagrus major, using glycolchitin and N-acetylchitooligosaccharides (GlcNAc(n), n=2-6) as substrates. High activity was observed at two pHs, such as 2.5 and 9.0, toward glycolchitin as seen in other insect chitinases, and also at both pH 2.5 and 5.0 even toward a short substrate, N-acetylc...