Histamine release induced by plant lectins was studied with emphasis on the carbohydrate specificity, external calcium requirement, metal binding sites, and mast cell heterogeneity and on the importance of antibodies bound to the mast cell membrane to the lectin effect. Peritoneal mast cells were obtained by direct lavage of the rat peritoneal cavity and guinea pig intestine and hamster cheek p...

Brekke, Johan Fredrik, William F. Jackson, and Steven S. Segal. Arteriolar smooth muscle Ca dynamics during blood flow control in hamster cheek pouch. J Appl Physiol 101: 307–315, 2006. First published February 2, 2006; doi:10.1152/japplphysiol.01634.2005.—Intracellular calcium concentration ([Ca ]i) governs the contractile status of arteriolar smooth muscle cells (SMC). Although studied in vit...

The purpose of this study was to examine whether neutral endopeptidase and angiotensin I-converting enzyme, two membrane-bound metalloenzymes that are widely distributed in the microcirculation, play a role in bradykinin-induced increase in vascular permeability in the hamster cheek pouch. Changes in vascular permeability were quantified by counting the number of leaky sites and by calculating ...

We tested the hypothesis that a parenchymally derived mediator is required for arterioles to exhibit oxygen sensitivity. To that end, the parenchyma was dissected and removed from around hamster cheek pouch arterioles, and the oxygen sensitivity of these 'aparenchymal arteriolar segments' was studied, either in vitro, after cannularion, or in situ. Arteriolar segments in situ with and without p...

The role of lime in the formation of reactive oxygen species (ROS), i.e., O2-., H2O2, and OH., from betel-quid components (extracts of areca nut and catechu) was investigated in vitro using a chemiluminescence technique and an assay for oxidative DNA damage involving analysis of 8-hydroxy-2'-deoxyguanosine. Of the various areca-nut extracts, the catechin fraction, at alkaline pH, was shown to b...

PURPOSE The purpose of this study was to evaluate the activity of CG53135 (FGF-20), a protein with in vitro mitogenic activity on epithelial and mesenchymal cells, in two in vivo models of oral mucositis (OM). EXPERIMENTAL DESIGN Radiation or concomitant chemotherapy/radiation-induced OM was elicited in hamsters. Activity of CG53135 was assessed at different doses and regimens in the models. ...

It is known that the progression of oral cancer is accompanied by changes in both tissue biochemistry and morphology. A multimodal imaging approach combining functional and structural imaging modalities could therefore provide a more comprehensive prognosis of oral cancer. This idea forms the central theme of the current study, wherein this premise is examined in the context of a multimodal ima...

The slow rolling motion of leukocytes along the walls of blood vessels mediated by specific receptor-ligand adhesion is important in inflammation and occurs in postcapillary venules over a wide range of wall shear stresses and vessel diameters. The ability of hydrodynamic collisions between cells to induce capture of free-stream leukocytes to a selectin-bearing surface under shear flow was stud...

Long-term mycoplasma (Strain HT) infection of cells of the FL line of human amnion has distinctly reduced their tumorproducing capacity in the cheek pouch of cortisonized weanling golden Syrian hamsters. The number of cells necessary for tumor production (TPDs0) was increased in the order 5 to 10 times, as compared to uninfected FL cells, for three different cell lines originating from independ...