The objective of this experiment was to determine the effect of a 5-day progesterone priming prior to a GnRH-PGF2alpha treatment on reproductive performance of anestrous goats. Thirty-six Mountain Black goats were randomly assigned in a 2 x 2 factorial arrangement and were administered intravaginally on day -12, either with 300 mg progesterone inserts (CGPE and CGP) or with 0 mg progesterone (G...

Two strains of the methylotrophic yeast Pichia pastoris were used to establish cyanophycin (multi-L-arginyl-poly-L-aspartic acid [CGP]) synthesis and to explore the applicability of this industrially widely used microorganism for the production of this polyamide. Therefore, the CGP synthetase gene from the cyanobacterium Synechocystis sp. strain PCC 6308 (cphA(6308)) was expressed under the con...

The diversity of angiotensin II (Ang II) actions implies multiple receptor subtypes. To characterize these subtypes in rat mesangial cells, we used the angiotensin subtype LA (ATu) antagonist losartan (DuP 753), the subtype 2/1B (AT2/AT,B) antagonist PD 123319, and the AT2 antagonist CGP 421L2A in radioreceptor and adenyiyi cyclase assays. In radioligand binding competition experiments, approxi...

Disruption of intracellular calcium initiates multiple cell-damaging processes, such as apoptosis. In normal cells, the levels of Ca(2+) are low in the mitochondria, whereas in apoptotic cells, Ca(2+) increases. Mitochondria uptake Ca(2+) via an inner membrane channel called the uniporter and extrude it into the cytoplasm through a Na(+)/Ca(2+) exchanger. Overload of Ca(2+) in the mitochondria ...

BACKGROUND Identification of clinically relevant oncogenic drivers in advanced cancer is critical in selecting appropriate targeted therapy. Using next-generation sequencing (NGS)-based clinical cancer gene assay, we performed comprehensive genomic profiling (CGP) of advanced cases of lung adenocarcinoma. METHODS Formalin-fixed paraffin-embedded tumors from 51 lung adenocarcinoma patients who...

Using a splanchnic nerve-spinal cord preparation in vitro that could spontaneously generate sympathetic nerve discharge (SND), we investigated the roles of intraspinal GABA(B) receptors in the regulation of SND. Despite an age-dependent difference in sensitivity, bath applications of baclofen (Bac; GABA(B)-receptor agonist) consistently reduced SND in a concentration-dependent manner. The drug ...

We study the spectrum of gravitational perturbations around a vacuum de Sitter brane in a 5D asymmetric braneworld model, with induced curvature on the brane. This generalises the stealth acceleration model proposed by Charmousis, Gregory and Padilla (CGP) which realises the Cardassian cosmology in which power law cosmic acceleration can be driven by ordinary matter. Whenever the bulk has infin...

We have assessed the functional correlates of common carotid artery (CCA) arterial geometry, derived by combining a measure of vascular mass (VM) with the wall-to-lumen (W/L) ratio in both untreated hypertensive (HT) and normotensive (NT; blood pressure <140/90 mm Hg) subjects of a broad age span (30 to 79 years) of both genders. Brachial systolic, diastolic, and pulse (SBP, DBP, PP) pressures;...

Hilbert space fragmentation is a novel type of ergodicity breaking in closed quantum systems. Recently, an algebraic approach was utilized to provide definition characterizing families Hamiltonian systems based on their (generalized) symmetries. In this paper we reveal simple connection between the aforementioned classification physical and coherence generation properties, quantified by generat...