1. A method for the separation of chromatin threads from the resting nucleus of leukemic cells has been described. 2. The isolation of the chromatin strands was accomplished by purely mechanical means, and the separation, including extraction and purification in the centrifuge, required only about 1 hour. 3. Evidence is presented to show that the chromatin strands are morphologically related to...

A rapid and sensitive single residual method (SRM) for the determination of ethephon which resemble a very polar, non-QuEChERS-amenable and plant growth regulator was developed. This method is applicable for determination of ethephon in vegetables, fruits and herbs. This method is simply based on extraction of ethephon with deionized water with using an equal amount of dichloro methane in order...

© 2008 American Institute of Physics, S-0031-9228-0809-020-0 The most difficult step in building a nuclear weapon is the production of fissile material. One can either make plutonium-239 in a nuclear reactor or enrich uranium to increase the abundance of its fissile isotope uranium-235. Historically, enrichment has been the more obscure of the two routes, but the recent spread of one technology...

In the production of biopharmaceuticals disk-stack centrifugation is widely used as a harvest step for the removal of cells and cellular debris. Depth filters followed by sterile filters are often then employed to remove residual solids remaining in the centrate. Process development of centrifugation is usually conducted at pilot-scale so as to mimic the commercial scale equipment but this meth...

Recent literature has reported that high concentrations of indicator bacteria such as fecal coliforms (FCs) were measured in anaerobically digested sludges immediately after dewatering even though low concentrations were measured prior to dewatering. This research hypothesized that the indicator bacteria can enter a non-culturable state during digestion, and are reactivated during centrifuge de...

Experimental proof is provided of an unconventional seismic design concept, which is based on deliberately underdesigning shallow foundations to promote intense rocking oscillations and thereby to dramatically improve the seismic resilience of structures. Termed rocking isolation, this new seismic design philosophy is investigated through a series of dynamic centrifuge experiments on properly s...

The vertebrate vestibular system detects linear (otolith organs) and angular (semicircular canals) acceleration. The function of the otolith system is twofold, 1: perception of linear acceleration of the head, and 2: assessment of the spatial orientation of the head relative to the vector of gravity. Because of the latter function, a change of gravity will affect the vestibular input which, in ...

The design of a Geosynthetic Reinforced Soil (GRS) wall for internal stability requires computing the reinforcement embedment length against pullout failure. The location of failure plane is important input for this design. The current FHWA design guidelines assume the location of failure plane based on Rankine theory. While this assumption holds true for conventional walls it is unconservative...

BACKGROUND Altitude and gravity changes during aeromedical evacuations induce exacerbated cardiovascular responses in unstable patients. Non-invasive cardiac output monitoring is difficult to perform in this environment with limited access to the patient. We evaluated the feasibility and accuracy of stroke volume estimation by finger photoplethysmography (SVp) in hypergravity. METHODS Finger ...

Mirror matter type dark matter can exist on the Earth’s surface, potentially in enhanced concentrations at various anomalous impact sites. Mirror matter fragments can draw in heat from the ordinary matter environment, radiate mirror photons and thereby cool the surrounding ordinary matter. We quantify this effect and suggest that it could be used to help locate mirror matter deposits. This meth...