A rapid microtechnic utilizing cellulose acetate membranes for the electrophoretic separation of LDH isoenzymes is described. The use of cellulose acetate for both migration and reagent application representsa relatively inexpensiveand reproducible method which avoids many of the inherent problems associatedwith agar gels. The technic can be used for the analysisof a relatively large number of ...

A short review with 49 references describes the electrospinninng (ES) process for polysaccharides, cellulose and chitosan, and their derivatives, including cellulose acetate and hydroxypropyl cellulose. A majority of applied studies adopted a two step-process, in which the cellulose acetate was used for the first ES process, followed by acetyl group removal to regenerate cellulose thin fibers. ...

The anomalous increase in cardiac glycogen during ketosis and the induction of a similar change by the infusion of ketone bodies or butyrate (4) suggest that a pathway allowing for the net accumulation of carbohydrate from fat may exist in heart. In an effort to assess this possibility, the conversion of n-butyrate-3-Cl4 to acetoacetate, P-hydroxybutyrate, glycogen, lactate, pyruvate, and fumar...

Serum containing normal and abnormal levels of alkaline phosphatase activity were assayed for total enzyme activity, then fractionated by electrophoresis on cellulose acetate membranes for 20 mm. The new substrate, phenolphthalein monophosphate, was employed to locate the isoenzymes on the cellulose acetate membranes and to measure their activity by eluting and scanning procedures. The sensitiv...

We describe for the first time a multi-antisera applicator to be used on cellulose acetate membranes with immunoelectrophoresis on a solid support. The new procedure overcomes previous shortcomings of immunoelectrophoresis on cellulose acetate membranes and permits rapid analyses of 11 samples on single “standard”-size membranes with a substantialsaving in antisera, celluloseacetate membranes, ...

A simple, sensitive staining procedure for the localization of leucine aminopeptidase isoenzymes after electrophoretic separation on cellulose acetate membranes is described. Separation of leucine aminopeptidase isoenzymes, using cellulose acetate membranes, does not appear to be a rewarding diagnostic procedure in the investigation of hepatobiliary or pancreatic disorders.

Short chain fatty acids (SCFA) are considered to be beneficial fermentation products in the gut by exerting trophic effects in non-transformed colon cells and by slowing proliferation and enhancing differentiation in colonic tumour cells. We have studied the further effects of SCFA on cellular events of early carcinogenesis, genotoxicity and cytotoxicity in rat distal colon cells. Cytotoxicity ...

Increased dietary fiber consumption has been associated with many beneficial effects, including amelioration of obesity and insulin resistance. These effects may be due to the increased production of short chain fatty acids, including propionate, acetate and butyrate, during fermentation of the dietary fiber in the colon. Indeed, oral and dietary supplementation of butyrate alone has been shown...

Fitch, Mark D., and Sharon E. Fleming. Metabolism of short-chain fatty acids by rat colonic mucosa in vivo. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G31–G40, 1999.—To determine the influence of substrate concentration and substrate interactions on short-chain fatty acid metabolism in vivo, a surgical procedure was established. Rats were surgically operated to cannulate a 5-cm segm...