statement of the problem: t cells have been shown to play a role in the etiopathogenesis of periodontal disease. b7-h3, a costimulatory molecule, is found to be associated with regulation of t cell function in some tumoral tissues, as well as autoimmune and inflammatory diseases. purpose: the aim of this study was to investigate the expression of b7-h3 molecule in healthy and diseased gingival ...

0079-6 doi:10. * Co Scienc Tokyo E-m 2.1. H labeling in early studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210 2.2. Uniform N labeling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210 2.3. Amino acid type-selective Cand ...

isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µm) to assess the changes in reactive oxygen species (ros) and lipid peroxidation leading to apoptotic hepatocyte cell death. the viability of hepatocytes was declined depending on the iron concentration. one hour incubation of the cells with 100 µm iron resulted in decreased...

PURPOSE: Monitoring of stem cells migrating or homing to the targets is essential for the success of stem cell-based therapies. For non-invasive magnetic resonance imaging (MRI) to serially track cell migration, recent efforts have focused on labeling cells with MR contrast agents, such as superparamagnetic iron oxide (SPIO) particles. The currently-available MR cell labeling techniques include...

We report on the surprising loss of transganglionic and retrograde labeling in the spinal cord of the rat after co-injection of the tracers wheat germ agglutinin-HRP (WGA-HRP) and choleragenoid toxin-HRP (CTB-HRP) into the sciatic nerve. Injection of WGA-HRP alone produced a pattern of transganglionic label consistent with transport by small-diameter primary afferent fibers. Small cell bodies w...

Questions of if and when protein structures change within cells pervade biology and include questions of how the cytoskeleton sustains stresses on cells--particularly in mutant versus normal cells. Cysteine shotgun labeling with fluorophores is analyzed here with mass spectrometry of the spectrin-actin membrane skeleton in sheared red blood cell ghosts from normal and diseased mice. Sheared sam...

BACKGROUND Articular cartilage has very limited intrinsic regenerative capacity, making cell-based therapy a tempting approach for cartilage repair. Cell tracking can be a major step towards unraveling and improving the repair process of these therapies. We studied superparamagnetic iron oxides (SPIO) for labeling human bone marrow-derived mesenchymal stem cells (hBMSCs) regarding effectivity, ...

UNLABELLED The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using (99m)Tc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized (99m)Tc-exametazime. METHODS Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized (99m)Tc-examet...

Fine neuronal morphology, such as dendritic spines, classically has been studied using the Golgi technique; however, Golgi staining is difficult to combine with other histological techniques. With the increasing popularity of fluorescent imaging, a number of fluorescent dyes have been developed that enable the coupling of multiple fluorescent labels in a single preparation. These fluorescent dy...

The changes in rate and location of cellular proliferation were analyzed to determine if localized areas of cell division were influencing shape changes in the chick thyroid. Pulse labeling with tritiated thymidine indicates that the gland's labeling index declines throughout its development. Initially, the thyroid placode has a lower labeling index than the neighboring pharyngeal epithelium. A...