نتایج جستجو برای: cell epitope

تعداد نتایج: 1693616  

Farzaneh Rabiee Hossein Baharvand Kamran Ghaedi Khadijeh Karbalaei Malihe Nazari Jahantigh Maryam Ostad Sharif Marzieh Nematollahi Mehran Miroliaei Mohamad Hossein Nasr Isfahani Shahnaz Razavi Somayeh Tanhaei

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a  chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...

2014
Leila Pishraft Sabet Tahereh Taheri Arash Memarnejadian Talat Mokhtari Azad Fatemeh Asgari Ramin Rahimnia Seyed Moayed Alavian Sima Rafati Katayoun Samimi Rad

BACKGROUND Hypervariability of HCV proteins is an important obstacle to design an efficient vaccine for HCV infection. Multi-epitope vaccines containing conserved epitopes of the virus could be a promising approach for protection against HCV. OBJECTIVES Cellular and humoral immune responses against multi-epitope DNA and peptide vaccines were evaluated in BALB/c mice. MATERIALS AND METHODS I...

Journal: :Journal of immunology 2015
Alexey P Martyushev Janka Petravic Andrew J Grimm Hamid Alinejad-Rokny Shayarana L Gooneratne Jeanette C Reece Deborah Cromer Stephen J Kent Miles P Davenport

CD8(+) T cells are important for the control of chronic HIV infection. However, the virus rapidly acquires "escape mutations" that reduce CD8(+) T cell recognition and viral control. The timing of when immune escape occurs at a given epitope varies widely among patients and also among different epitopes within a patient. The strength of the CD8(+) T cell response, as well as mutation rates, pat...

Journal: :Cancer research 2009
Lisa M Ebert Yu Chih Liu Craig S Clements Neil C Robson Heather M Jackson Jessica L Markby Nektaria Dimopoulos Bee Shin Tan Immanuel F Luescher Ian D Davis Jamie Rossjohn Jonathan Cebon Anthony W Purcell Weisan Chen

The tumor antigen NY-ESO-1 is a promising cancer vaccine target. We describe here a novel HLA-B7-restricted NY-ESO-1 epitope, encompassing amino acids 60-72 (APRGPHGGAASGL), which is naturally presented by melanoma cells. The tumor epitope bound to HLA-B7 by bulging outward from the peptide-binding cleft. This bulged epitope was not an impediment to T-cell recognition, however, because four of ...

Journal: :Journal of immunology 2002
Daniel C Douek Michael R Betts Jason M Brenchley Brenna J Hill David R Ambrozak Ka-Leung Ngai Nitin J Karandikar Joseph P Casazza Richard A Koup

Escape from the CD8(+) T cell response through epitope mutations can lead to loss of immune control of HIV replication. Theoretically, escape from CD8(+) T cell recognition is less likely when multiple TCRs target individual MHC/peptide complexes, thereby increasing the chance that amino acid changes in the epitope could be tolerated. We studied the CD8(+) T cell response to six immunodominant ...

2003
H Shiku Lijie Wang Yoshihiro Miyahara Takuma Kato Linan Wang Takumi Aota Kagemasa Kuribayashi Hiroshi Shiku

In this study, we identified a c-erbB-2/HER2/neu (HER2)-derived Th epitope (HER216-30) and examined the role of Th epitopes in HER2-specific CD8+ T cell induction and in vivo tumor eradication, with a particular emphasis on the role of tumor cell-derived Th epitopes. Immunization of BALB/c mice using a mixture of Th epitope HER216-30 and CTL epitope HER263-71 administered subcutaneously with mu...

Journal: :Biomedical journal 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic involving so far more than 22 million infections and 776,157 deaths. Effective vaccines are urgently needed to prevent SARS-CoV-2 infections. No have yet been approved for licensure by regulatory agencies. Even though host immune responses beginning be unravelled, effective clearance of virus will depend on both ...

Journal: :The Journal of Experimental Medicine 1994
M Guéguen W E Biddison E O Long

An alternative pathway for class I-restricted antigen presentation has been suggested on the basis of peptides bound to HLA-A2 molecules in cells lacking the transporter for antigen presentation (TAP). Most of these peptides were derived from signal sequences for translocation into the endoplasmic reticulum (ER). However, it is not known whether these peptides can be presented to T cells. The h...

Journal: :Molecular medicine reports 2016
Xuelei Liu Hui Zhao Wenyan Cao Yumei Liu Chuntao Zhang Xi Lan Shanshan Peng Hao Wen Jianbing Ding Xiumin Ma

Echinococcosis is a zoonotic parasitic disease affecting humans and other mammals, which is mainly caused Echinococcus at larval stages. It is predominantly endemic in Chinese pasture regions, including Xinjiang, Qinghai, Gansu and Ningxia. The aim of the present study was to predict the T‑ and B‑combined epitopes of Echinococcus granulosus (Eg). ferritin, and to analyze its secondary structure...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2004
Kwong-Yok Tsang Claudia Palena James Gulley Philip Arlen Jeffrey Schlom

PURPOSE MUC-1/DF-3 remains an attractive target for vaccine therapy. It is overexpressed in the majority of human carcinomas and multiple myeloma. Clinical trials using MUC-1-based vaccines have demonstrated safety, clinical responses, and the induction of T-cell responses directed against MUC-1. Previous studies in experimental models and in clinical trials have demonstrated that altering the ...

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