نتایج جستجو برای: cell cultivation
تعداد نتایج: 1712921 فیلتر نتایج به سال:
The objective of this research was to investigate the influence of the over-expression of recombinant interferon-g during high cell density cultivation on cellular characteristics of recombinant E. coli. Batch and fed-batch culture techniques were employed to grow Escherichia coli BL21 for production of human gamma-interferon in pET expression system. Final cell densities in batch and fed-batch...
Optimization of Equine Influenza Strain A/equine/Otar/764/07 (H3N8) Cultivation in MDCK Cell Culture
The paper presents the results of a study of cultural characteristics of the strain A/equine/Otar/764/07 (H3N8) of equine influenza virus isolated from sick horse during the outbreak of equine influenza epizooty in the Republic of Kazakhstan. The studies determined the optimal culture conditions for equine influenza virus strain A/equine/Otar/764/07 (H3N8) in MDCK cell culture. The optimal infe...
This study is pertaining to the production of penicillin G acylase (PGA) by Bacillus subtilis 8105MU331 in which PGA gene, under the control of thermal-induced promoter, was integrated. The key process parameters including induced-temperature, induced-time, and culture temperature were optimized in flask culture. A three-stage cultivation process was developed for PGA production with the expres...
Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact...
We demonstrated the feasibility of fedbatch operation using Clostridium acetobutylicum suspension culture as a biocatalyst for the continuous production of hydrogen. The optimum operating pH and temperature of the current cultivation system for hydrogen production were pH 6.0 and 37 degrees C, respectively. The volumetric loading of the bioreactor for hydrogen production can be as high as 650 m...
Background Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease. Packed-bed bioreactors have been used for the cultivation and production of a wide range of cell lines and biologics including MAbs. The principle behind a Packed-bed bioreactor is that the cells are being immobilized within a suitable stationary matrix which is represented...
Introduction The Quality by Design (QbD) approach shows significant benefit in classical pharmaceutical industry and is now on the cusp to a stronger influence on biopharmaceutical applications. Monitoring the critical process parameters (CPP) applying process analytical technologies (PAT) during biotechnological cell cultivations is of high importance in order to maintain a high efficiency and...
glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 pdo). here we report a newly isolated bacterial strain ba11 from soil, capable of fermenting glycerol to 1,3 pdo, and has been identified to be a strain of klebsiella pneumoniae. strain ba11 was fast growing showing peak 1,3 pdo production in 6 h of cultivation with productivity of 1.2 g/l-h witho...
The ability of the bacterium, Rhodococcus UKM-P, to degrade phenol was studied in shake flask culture. The effect of the cultivation parameters (temperature, pH, type and concentration of nitrogen source and salt concentration) on growth of the bacterium and phenol degradation was evaluated. The highest cell growth and the amount of phenol degraded (0.5 g/L) were observed in optimized cultivati...
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