organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. the aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. the spinal cord was sliced and incubated into culture medium. the mtt assay was carried out to a...

We have developed high throughput fluorescence cell imaging methods to screen chemical libraries for compounds with effects on diverse aspects of cell physiology. We describe screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway. Each of these screens yielded specific inhibitors for research use, and the mitosis screen identified Eg5...

Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell ...

background: although cockroaches are mechanical vector of many pathogens, still biological transmission of pathogens via these insects is controversial. more covenant immune defense in the hemolymph of cockroaches may take this ad­vantage to block any adaptation of pathogens regarding to biological transmission. lectin molecules in hemolymph of in­sects play as mediators of nonself recognition,...

Primary effusion lymphoma-like lymphoma (PEL-LL) is a rare B-cell lymphoma that the etiology remains unclear. We describe a case of PEL-LL with a pleuropericardial effusion. Diagnosis required long period of time as it followed a unique progress of disappearance and recurrence of the body cavity effusion. We finally had a diagnosis of B-cell lymphoma by the immunocytochemistry of effusion using...

The two ends of spectrum benign to malignant pose no problem, however in the middle lies a “no man’s land” where wise men tread cautiously. Surgical Pathology and Cytopathology are the main stay in the tumor diagnosis. However, newer technologies like-Immunohistochemistry, Molecular diagnosis, Flowcytometry and Tumor markers introduced in the past decade, have contributed immensely towards achi...