Previous studies in this laboratory showed that the reduction of diphosphopyridine nucleotide with molecular hydrogen, as catalyzed by extracts of C~ostridium kluyveri, involves the participation of at least two protein components (1, Z), one of which is ferredoxin (3), and of at least two cofactors obtainable from boiled cell extracts (4). The heat-stable cofactors were separated by column chr...

Investigation of the acylation scope of carbohydrates by metalloprotease thermolysin immobilized on Celite as biocatalyst has been carried out. The reactions were performed in DMSO, a good solvent for carbohydrates, where the enzyme has previously shown its activity in transesterifications of sucrose, maltose and maltose-containing oligosaccharides. Surprisingly, no reaction was observed for gl...

The objective of present study was to evaluate the response of the mung bean seeds of ‘Sonali B1’ variety primed with chitosan in four different concentrations (0, 0.1%, 0.2% and 0.5%) under salinity stress of five different concentrations (i.e., 0, 4, 6, 8 and 12 dS*mm) and halotolerance pattern by applying Celite as matrix at three different moisture levels (5%, 10% and 20%). Improved germina...

To help us identify appropriate techniques and laboratories for measuring hormones, we studied the variability and reproducibility of assay measurements of androstanediol glucuronide, androstenedione, dehydroepiandrosterone (DHEA), DHEA sulfate, dihydrotestosterone, testosterone, androstanediol, androsterone glucuronide, and androsterone sulfate for five men. Four sets of two aliquots from each...

tion as measured by the hydroxamate reaction did not change appreciably, and furthermore, the amount of oxaloacetate synthesized was almost twice the amount of acetyl-CoA added. In other experiments enzymatic measurements of acetyl-CoA concentration gave similar results. The indirect role of acetyl-CoA is further confirmed by experiments shown in Table III. Oxaloacetate was formed from various ...

This paper describes a method for the isolation of strains of tobacco mosaic virus (TMV) which consist of infective particles that are labile under conditions where ordinary TMV is extremely stable. Two such strains have been isolated, PML and PM2, which are distinctly different in their properties. Evidence will be presented to demonstrate that tobacco plants infected with strain PM1 fail to s...