macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

Our group has been making effort to collect mouse full-length cDNA clones as large scale Mouse cDNA project. It is aiming at collecting all kind of expressed full-length Mouse cDNA clones. The first phase of the project is to make cataloged non-redundant cDNA clone bank. It is necessary for analysis of sequence data and further applications of cDNA clones. We sequence 3’-end part of all cDNA cl...

We describe the use of a synthetic primer to select a cDNA recombinant clone containing H5 coding sequences. The strategy used was as follows: 1. Prepare oligo(dT) cellulose-bound mRNA from chicken reticulocytes and select 11S-18S material from sucrose gradients. 2. Use this RNA fraction both to prepare a cDNA library and as a template for H5-specific cDNA synthesis using a synthetic primer. 3....

The size of the DNA product synthesized by RNA-directed DNA polymerase (isolated from avian myeloblastosis virus) was found to be important for complementary DNA (cDNA)-mRNA hybridization reactions. Incomplete cDNA to rabbit reticulocyte globin mRNA formed poor hybrids and presumably lacked sequences needed for hybridization. The size of the cDNA synthesized was influenced by the reaction condi...

Both the frequency and intensity of drought events are expected to increase, with unresolved alterations peatland methane cycling involved microbial communities. While existing studies have assessed effects via experimental approaches under controlled conditions, our knowledge, no examined in-situ natural in restored temperate fens. In this study, we used quantitative polymerase chain reaction ...

BACKGROUND Genome annotation projects, gene functional studies, and phylogenetic analyses for a given organism all greatly benefit from access to a validated full-length cDNA resource. While increasingly common in model species, full-length cDNA resources in aquaculture species are scarce. METHODOLOGY AND PRINCIPAL FINDINGS Through in silico analysis of catfish (Ictalurus spp.) ESTs, a total ...

seth4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of arabidopsis thaliana. this fragment was pcr amplified using kod hi fi dna polymerase enzyme. this fragment was cloned into pgbkt7 bate vector and transformed e. coli dh5? cells containing vector were selected on lb medium containing kanamycin. finally, pgbkt7-seth4 bate was transformed into yeast ...

cDNA species coding for α-methylacyl-CoA racemase were cloned from rat and mouse liver cDNA libraries and characterized. The rat liver λgt11 cDNA expression library was screened with anti-racemase IgG [Schmitz, Albers, Fingerhut and Conzelmann (1995) Eur. J. Biochem. 231, 815–822]. Several fulllength clones were obtained that contained an open reading frame of 1083 bp, coding for a protein of 3...