Background/Aim. The fact that lung carcinomas, like other solid tumours, can be immunogenic, may have a substantial prognostic value in non-small cell cancer. Specific cytotoxic T-lymphocytes demonstrated most patients with primary tumours of different histological types. Two main groups participate the coupled recognition tumour-specific antigens - (CD8+, cluster differentiation 8) and helper ...

CD8(+) T cell anergy is a critical mechanism of peripheral tolerance, poorly investigated in response to immunotherapy. Here, using a pancreatic islet allograft model and CD3 antibody therapy, we showed, by single cell gene profiling, that intragraft CD8(+) lymphocytes coexpressing granzyme B and perforin were selectively depleted through the Fas/FasL pathway. This step led to long-standing ane...

SOCS1(-/-) mice accumulate within the thymus and periphery CD8(+) lymphocytes that express memory cell markers and display heightened in vitro responses to common gamma-chain cytokines. To investigate whether dysregulated homeostasis of T lymphocytes and acquisition of memory phenotype by CD8(+) cells in SOCS1(-/-) mice were mediated by IL-7 and/or IL-15 in vivo, we have generated SOCS1(-/-)IL-...

The relationship between the number of circulating CD4+ T cells and the presence of particular CD8+ T cell subsets was analyzed by flow cytometry on PBL from asymptomatic HIV-1-infected patients whose specimens were collected every 2 mo for a total period of 32 mo. Only slight variations were detected in the absolute number of lymphocytes and percentage of CD3+ lymphocytes, whereas both CD4+ an...

We previously showed that HIV infection leads to expansion of a rapidly proliferating pool (s(1)) of CD4 and CD8 T lymphocytes. In the current study, we used in vivo labeling with bromodeoxyuridine to characterize the kinetics of naive, memory, and activated (HLA-DR(+)/CD38(+)) subpopulations of CD4 and CD8 T lymphocytes, and to examine the relationship between kinetic parameters and baseline C...

Abstract Backgrounds The present study explored the viability of bovine milk macrophages, their intracellular production reactive oxygen and nitrogen species (RONS), phagocytosis Staphylococcus aureus , as well profile lymphocytes, from healthy udder quarters infected by Corynebacterium bovis . included 28 12 dairy cows 20 C. 10 cows. percentages macrophages lymphocytes were identified flow cyt...

Background: &beta-glucans are glucose polymers with a variety of stimulatory effects on the immune system. The objective of this study was to evaluate the immune-enhancing activities of low molecular weight gamma irradiated &beta-glucan (I&beta-g) extracted from Pleurotus ostreatus in response to Pseudomonas aeruginosa infection in rats. Materials and Methods: &beta-glucan (&beta-g) powder was ...

Background: Candida albicans is one of the most important opportunistic pathogens that suppress immunologic mechanisms of the host. It is speculated that structural and secretory proteins of C. albicans have immunomodulatory effects in cancer. Objective: To evaluate the effects of C. albicans structural and secreted proteins on intratumoral CD4/CD8 ratio as well as the survival rate in BALB/c t...

Background Cytotoxic CD8 T lymphocytes (CTL) mediate target cell killing of tumor cells. New technological advances in multi-parameter flow cytometry have enabled the detection and enumeration of antigen-specific CD8 T cells (MHC-class I dextramer staining) in peripheral blood. A new dextramer based IVD test has been approved in Europe for the quantification of CMV specific CD8 T cells in perip...

Human Ag-specific CD8(+) T lymphocytes are heterogeneous and include functionally distinct populations. In this study, we report that at least two distinct mechanisms control the expansion of circulating naive, memory, and effector CD8(+) T lymphocytes when exposed to mitogen or Ag stimulation. The first one leads to apoptosis and occurs shortly after in vitro stimulation. Susceptibility to cel...