Changes in ATP-induced increase in [Ca2+](i) during collecting duct ontogeny were studied in primary monolayer cultures of mouse ureteric bud (UB) and cortical collecting duct (CCD) cells by Fura-PE3 fluorescence ratio imaging. In UB (embryonic day E14 and postnatal day P1) the ATP-stimulated increase (EC(50) approximately 1 microM) in fluorescence ratio (DeltaR(ATP)) was independent of extrace...

In this article a comparative study for modeling and optimization of 2-methylpropane-2-thiol removal from contaminated soil by ultrasound is investigated. Central Composite Design (CCD) and artificial neural network (ANN) were utilized and compared to each other in order to obtain appropriate predicting model with respect to sonication power (w), sonication time (min) and water/reactor volume r...

We describe the overall performance of the STIS CCD after HST Servicing Mission #4 and the associated updates to calibration reference files. Most aspects of CCD performance are found to be fairly consistent with extrapolations of the trends seen prior to the failure of STIS in August 2004. The CCD gain value for the CCDGAIN = 4 setting has been redetermined using net count ratios of standard s...

ROMK potassium channels are present in the cortical collecting duct (CCD) of the kidney and serve as apical exit pathways for K+ secretion in this nephron segment. K+ secretion in the CCD is regulated by multiple factors. In this study, we show that syntaxin 1A, but not syntaxin 3 or 4, inhibited whole cell ROMK currents in Xenopus laevis oocytes. Syntaxin 1A, but not syntaxin 3 or 4, interacte...

BACKGROUND Cleidocranial dysplasia (CCD) is an autosomal dominant skeletal disorder characterised by hypoplastic or absent clavicles, increased head circumference, large fontanels, dental anomalies and short stature. Although CCD is usually caused by mutations leading to haploinsufficiency of RUNX2, the underlying genetic cause remains unresolved in about 25% of cases. METHODS Array comparati...

Background: Hippocampal atrophy is a key pathologic and magnetic resonance imaging (MRI) feature of human Alzheimer’s disease (AD). has not been documented via MRI in canine cognitive dysfunction (CCD), which considered as the dog model AD.Aim: The purpose this retrospective comparative volumetric study was to compare total hippocampal volumes between successfully aging (control) dogs diagnosed...

In this paper we present methods for characterizing CCD cameras. Interesting properties are linearity of photometric response, signal-to-noise ratio (SNR), sensitivity, dark current and spatial frequency response (SFR). The techniques to characterize CCD cameras are carefully designed to assist one in selecting a camera to solve a certain problem. The methods described were applied to a variety...