Due to the lower DMI, the IH200 heifers had lighter final weight and lower ADG on a live basis (P<.01) than the IHFH heifers, but these differences were reduced on a carcass-adjusted basis. The IH200 heifers had significantly improved feed conversion compared to FH and R200 (P = 0.01) and tended to have better feed conversion than IHFH (P = 0.14) on a carcass-adjusted basis. The IH200 heifers h...

INTRODUCTION Despite the scientific consensus on the benefits that breastfeeding provides for the mother, the baby, the family and the environment, and also the recommendation to breastfeed exclusively for six months, this practice is far from being achieved. OBJECTIVE To analyze the factors associated with exclusive breastfeeding (EBF) for at least six month, as opposed to weaning up to the ...

Results Decreased turnover of fat cells and their lipid contents is associated with insulin resistance, dyslipidemia and altered metabolic function of fat cells. Low fat cell turnover leads to adipose hypertrophy which is linked to insulin resistance in subcutaneous fat and to dyslipidemia in visceral fat. A transcription factor (EBF-1) regulates hyperplasia/hypertrophy in human adipose tissue ...

Zfp423 is a 30 zinc finger transcription factor that forms regulatory complexes with EBF family members and factors targeted by canonical signaling pathways. Zfp423 mutations produce a range of developmental abnormalities in mice and humans related to the ciliopathies. Surprisingly, computational analysis of clustered Zfp423 and partner motifs in conserved genomic sequences predicts enrichment ...

OBJECTIVE To examine the relationship between family structure and the prevalence of exclusive breastfeeding (EBF) in Nicaragua. MATERIAL AND METHODS A subset of the Nicaraguan Demographic and Health Survey conducted in 1998 was obtained by selecting all last-born infants less than 4 months old at the time of the survey. Weighted chi-squared and multivariate logistic regression analyses were ...

Method 1,303 exclusively breastfeeding (EBF) infants were recruited to the EAT Study at 3 months of age and randomized to either EBF until around 6 months of age (Standard Introduction Group SIG) or early introduction of 6 allergenic foods (cow’s milk, egg, fish, wheat, sesame and peanut) alongside breastfeeding (Early Introduction Group EIG). Data from the UK 2010 Infant Feeding Survey (IFS) w...

Long- and short-term stability testing of the analyte is one of the key parameters in bioanalytical method validation in support of pharmacokinetics. However, for immunogenicity testing, the scientific rationale for long- and short-term stability testing on quality control samples most often spiked with polyclonal antibody raised in a different species should be questioned. Therefore, the Europ...

Total parenteral nutrition (TPN) administration in a mouse model leads to a local mucosal inflammatory response, resulting in a loss of epithelial barrier function (EBF). Although, the underlying mechanisms are unknown, a major contributing factor is a loss of growth factors and subsequent critical downstream signaling. An important component of these is the p-Akt pathway. An additional contrib...

The CRISPR/Cas9 system has ushered in a new era of targeted genetic manipulations. Here, we report the use of CRISPR/Cas9 to induce double-stranded breaks in the genome of the sea squirtCiona intestinalis. We use electroporation to deliver CRISPR/Cas9 components for tissue-specific disruption of the Ebf (Collier/Olf/ EBF) gene in hundreds of synchronizedCiona embryos. Phenotyping of transfected...