نتایج جستجو برای: catalytic determination
تعداد نتایج: 328512 فیلتر نتایج به سال:
A highly sensitive catalytic spectrofluorimetric method for the determination of hydrogen peroxide and peroxidase is presented. This is based on catalytic effect of peroxidase on the oxidation of para-acetylaminophenol, a non – fluorescent compound to fluorescent probe by hydrogen peroxide in slightly basic medium and the reaction mechanism was investigated. The reaction was followed spectroflu...
Conditions for accurate measurement of catalytic activity of aspartate aminotransferase and alanine aminotransferase in human serum have been reinvestigated. The basic variables (kind of buffer, buffer concentration, pH, ion effects, and the influence of pyridoxal-5-phosphate) can now be considered optimized. On this basis, the kinetic parameters of both aminotransferases were determined, i.e.,...
We have identified ribose 2'-hydroxyl groups (2'-OHs) that are critical for the activity of a trans-cleaving delta ribozyme derived from the antigenomic strand of the hepatitis delta virus. Initially, an RNA-DNA mixed ribozyme composed of 26 deoxyribo- (specifically the nucleotides forming the P2 stem and the P4 stem-loop) and 31 ribonucleotides (those forming the catalytic center) was engineer...
Serial femtosecond crystallography (SFX) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. Crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. Copper nitrite r...
SIRT1, the founding member of the mammalian family of seven NAD(+)-dependent sirtuins, is composed of 747 amino acids forming a catalytic domain and extended N- and C-terminal regions. We report the design and characterization of an engineered human SIRT1 construct (mini-hSIRT1) containing the minimal structural elements required for lysine deacetylation and catalytic activation by small molecu...
A thin film of epinephrine (EP) was electrochemically deposited on the surface of glassy carbon electrode previously activated in NaHCO3 solution. The cyclic voltammograms of the modified electrode indicate that the surface confined EP are strongly dependent on the solution pH, as expected for quinone/hydroquinone functionalities. The EP-modified glassy carbon electrode exhibited...
Type of packings and characteristics of their geometry can affect the flow behavior in the reactive distillation columns. KATAPAK SP is one the newest modular catalytic structured packings (MCSP) that has been used in the reactive distillation columns, recently. However, there is not any study on the hydrodynamics of this packing by using computational fluid dynamics. In the present work, a 3D ...
ATP-dependent Lon proteases belong to the superfamily of AAA+ proteins. Until recently, the identity of the residues involved in their proteolytic active sites was not elucidated. However, the putative catalytic Ser-Lys dyad was recently suggested through sequence comparison of more than 100 Lon proteases from various sources. The presence of the catalytic dyad was experimentally confirmed by s...
Naphthimidazolium based monocationic chemodosimeters CD-1 and CD-2 undergo cyanide mediated catalytic transformation in the presence of cyanide ions (0.01% to 1% of CD-1/CD-2 concentrations) with a turnover number from 70 to 360. These chemodosimeters can detect as low as 0.5 nM and 1 nM cyanide ions under nearly physiological conditions (HEPES buffer-DMSO (5%), pH 7.4). The structures of CD-1 ...
Kinetic studies were carried out on alkaline phosphatase (ALP) extracted from the liver of Agama agama lizard. Incubation of ALP extract with para – nitrophenyl phosphate formed the basis for the determination of enzyme activity. Spectrophotometric method was used to assay the enzyme, and the kinetic constants: Maximum velocity (Vmax) and Michaelis Menten constant (Km) were calculated. The Km a...
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