Fish protein hydrolysates were prepared from Cuttlefish (Sepia pharaonis) muscle using alcalase and protamex methods. By conducting the Cuttlefish protein hydrolysate using alcalase (CPHA), it showed higher degrees of hydrolysis, proximate composition, yield, emulsifying activity index (EAI), emulsion stability index (ESI), foaming activity index (FAI), solubility, water holding capacity (WHC) ...

Extracellular tannase production by Trichophyton rubrum has been evaluated using submerged fermentation (SmF) at different abiotic factors like pH, temperatures and biotic factors like carbon, nitrogen and concentration of gallic acid. T. rubrum produced the highest tannase level at a temperature of 30°C where the enzyme production was 23.52 U/mL and pH of 5.5 where enzyme production was 32.62 ...

Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% ...

The cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132 was fused to the secretive lipase signal peptide of B. subtilis. This leads to an efficient secretion of the recombinant enzyme into the culture medium of E. coli as an active and soluble form contrasting with the native construction leading to a periplasmic production. In order to enhance the yield of CGTase production...

A selective agar medium (medium J(1)) is proposed for the quantitative enumeration of egg yolk-positive (EYP) and egg yolk-negative (EYN) Staphylococcus pyogenes from the digestive tract and feces of the rat. This medium, buffered at pH 5.0, is composed of acid casein hydrolysate and yeast extract with 7.5% sodium chloride, 1.6% sodium pyruvate, 0.0008% 2,3,5-triphenyltetrazolium chloride (TTC)...

The hypothesis for this study is that weaning to an extensively hydrolyzed infant formula will decrease the incidence of type 1 diabetes (T1D), as it does in all relevant animal models for the disease. This will be tested in children who carry risk-associated human leukocyte antigen genotypes and have a first-degree relative with T1D. The trial will use a double-blind, prospective, placebo-cont...

In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media significantly influenced the germination frequency. Seeds at 13 months after pollination cultured on 3% sucrose...

Two strains (E-26 and B) of Escherichia coli were examined for their growth response in a medium containing 0.75 yo (w/v) NaCl, 1 yo (w/v) L-lysine hydrochloride, 2 % (w/v) glucose, 2.4 % (w/v) nutrient broth, and 5 % (w/v) casein hydrolysate. Strain E-26 grew as a rod which was slightly larger than when it was grown in a defined medium. Strain B grew as a filamentous and branched form; while i...

Evaluation of four botanicals Vernonia amygdalina L., Bryophyllum pinnatum L., Eucalyptus globules Labill and Ocimum gratissimum Kurz (Clocimum) for chemical and nutritional properties was conducted at Ago Iwoye in South-western Nigeria. The results from the investigation showed that V. amygdalina contained large quantity of Thiamine, Pyridoxine, Ascorbic acid, Glycine, Cysteine and Casein hydr...

Several factors which regulate the synthesis of enterotoxin B were examined in Staphylococcus aureus S-6 and in its heme-requiring mutant S-6H2. The kinetics of enterotoxin B synthesis during anaerobic growth were identical to those observed under aerobic conditions; extracellular enterotoxin accumulated in the medium during the transition between exponential and stationary phase growth. Strain...