Nepenthes mirabilis (Lour) Druce is a rare ornamental plant and has high economic value so it needs to be preserved. This study aimed analyze effect of sucrose BAP concentrations their optimal interactions in the vitro multiplication mirabilis. Explants form plantlets from Laboratory Plant Tissue Culture, Department Biology, Semarang State University. The treatment was carried out by adding mur...

An efficient in vitro regeneration protocol was developed via callus from the explants of leaf and internode Coccinia cordifolia (L.) Cogn., an important medicinal climber. Among different combinations auxins cytokines used, BAP with NAA were found highly effective for induction derived disc explants. Maximum formation rates recorded on MS 1.0 mg/l 0.1 both The best organogenesis obtained 2.0 0...

an important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. nowadays, the use of compounds such as essential oils (eos) and nanoparticles is applicable in microbiology studies. the main objective of this experiment was to study the s...

many factors may influence the efficiency of somatic embryogenesis. this capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. the effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mm) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

Objective(s): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression.  In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. Materials and Methods:Expression of CD11b and CD...

PURPOSE To analyze the in vitro Müller cell modifications in an organotypic culture of porcine neuroretina in response to the addition of a blood-derived mononuclear fraction (MNF; monocytes and lymphocytes) as a source of macrophages. METHODS Control and MNF-stimulated neuroretinal explants were examined at 3, 6, and 9 days of culture. Specimens were processed for epoxy-resin embedding and c...

Previously, we reported that primary cultures of human stromal vascular (SV) cells containing newly differentiated adipocytes treated with 30 uM trans-10, cis-12 CLA for 24 h had greater gene expression and protein secretion of IL-6 and IL-8 than vehicle controls (Brown et al. 2004). In addition, cultures treated with 30 uM trans-10, cis-12 CLA had decreased mRNA levels of PPARγ2 and several of...