A 39-nucleotide spliced leader (SL) is joined to the 5' ends of trypanosome mRNAs in a bimolecular or trans-splicing process. The SL in Trypanosoma cruzi is transcribed as an approximately 110-nucleotide RNA (SL-RNA or SL primary transcript) bearing the 39-nucleotide SL at the 5' end. The SL-RNA is 5' capped by a guanylyltransferase activity prior to trans-splicing and trypanosome mRNAs thus ob...

Flaviviruses are small, capped positive sense RNA viruses that replicate in the cytoplasm of infected cells. Dengue virus and other related flaviviruses have evolved RNA capping enzymes to form the viral RNA cap structure that protects the viral genome and directs efficient viral polyprotein translation. The N-terminal domain of NS5 possesses the methyltransferase and guanylyltransferase activi...

RNA cap binding proteins have evolved to specifically bind to the N7-methyl guanosine cap structure found at the 5' ends of eukaryotic mRNAs. The specificity of RNA capping enzymes towards GTP for the synthesis of this structure is therefore crucial for mRNA metabolism. The fact that ribavirin triphosphate was described as a substrate of a viral RNA capping enzyme, raised the possibility that R...

PURPOSE To (1) study the injury and healing activity of the pulp tissue to calcium hydroxide [Ca(OH)2], resin composite (RC) and resin-modified glass-ionomer (RMGI) materials when used as direct pulp capping agents, and (2) compare the incidence of healing defects between these materials. METHODS 135 Class V pulp exposed cavities were prepared in non-human primate teeth. Direct pulp capping w...

Fluorescent derivatives of gangliosides were prepared by oxidizing the sialyl residues to aldehydes and reacting them with fluorescent hydrazides. When rhodaminyl gangliosides were incubated with lymphocytes, the cells incorporated them in a time- and temperature-dependent manner. Initially, the gangliosides were evenly distributed on the cell surface but were redistributed into patches and cap...

Glycolipid-enriched membrane (GEM) domains, or lipid rafts, function in signaling in immune cells, but their properties during Ag presentation are less clear. To address this question, GEM domains were studied using fluorescence cell imaging of mouse CH27 B cells presenting Ag to D10 T cells. Our experiments showed that APCs were enriched with GEM domains in the immune synapse, and this occurre...

OBJECTIVE To evaluate the success rate of a calcium hydroxide-based direct capping agent in permanent teeth. METHOD AND MATERIALS A total of 1,075 permanent teeth were treated in this study. All patients received direct pulp capping with a calcium hydroxide-based agent. Inclusion criteria were teeth with healthy pulps, pulps with signs of reversible pulpitis, and a pulp chamber roof opening s...

Ultrastructural analyses have revealed striking similarities between Concanavalin A capping and phagocytosis in leukocytes. Both processes involve extensive membrane movement to form a protuberance or pseudopods; a dense network of microfilaments is recruited into both the protuberance and the pseudopods; microtubules are disassembled either generally (capping) or in the local region of the pse...

Cellular enzymes that cap, splice, and polyadenylate eukaryotic pre-mRNAs are targeted in vivo to the nascent chains synthesized by RNA polymerase II (pol II). Placing a mammalian pol II transcription unit under the control of a pol III promoter results in a failure to cap, splice, or polyadenylate the transcript (1, 2). How is pre-mRNA ‘‘identity’’ established? Do the various mRNA processing e...

introduction: the aim of the present investigation was to compare the efficacy of mineral trioxide aggregate (mta) and 0.2% chlorhexidine (chx) mixture to pure mta, as a pulp capping material. methods and materials: the pulp of 24 lateral incisors and canines from four dogs were exposed and capped either with mta or mta+0.2% chx. after 2 months the animals were sacrificed and the teeth were pre...