During post-embryonic development, roots can be initiated by a programmed developmental order or by environmental and wound stimulation (Bellini et al., 2014; Xu and Huang, 2014; Birnbaum, 2016; Ikeuchi et al., 2016; Kareem et al., 2016; Lup et al., 2016; Rellan-Alvarez et al., 2016; Steffens and Rasmussen, 2016). De novo root regeneration (DNRR) is a type of plant regeneration to produce adven...

OBJECTIVE To investigate the relationship among plantar foot pressure, plantar subcutaneous tissue thickness, severity of neuropathy (vibration perception threshold [VPT]), callus, and BMI in a large group of neuropathic diabetic patients at risk of foot ulceration. RESEARCH DESIGN AND METHODS A total of 157 diabetic neuropathic patients (VPT >25 V) without either peripheral vascular or ulcer...

Background Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materi...

Treatment of large bone defects and incurable fractures is a challenging clinical problem. A novel approaches in engineering the use adipose-derived stem cells adipose tissue. This study aimed to analyse impact autograft on process regeneration surgically created critical size defect (CSD) hen ulna. In this study, 30 laying hens at age 14 months were randomly divided into three groups: control ...

The capacity of plants to regenerate is impressive. Plant cells can undergo somatic embryogenesis in culture, forming an embryo from a single cell or a group of somatic cells. In addition, plants exhibit de novo organogenesis, in which new organs and even entire plants are produced from other organs upon wounding. Almost as remarkable as the phenomenon of plant regeneration is how little we kno...

Brinjal (Solanum melongena L.) var. Mattu Gulla (MG) and var. Perampalli Gulla (PG) are unique varieties with distinct flavour cultivated in Udupi, Karnataka State, and are exposed to several biotic and abiotic stresses. An efficient and reproducible in vitro regeneration method is required to expedite the manipulation of these brinjal varieties to cope up with stress by tissue culture and gene...

N(6)-methyladenosine (m(6)A) is the most prevalent internal modification present in mRNAs of all higher eukaryotes. With the development of MeRIP-seq technique, in-depth identification of mRNAs with m(6)A modification becomes feasible. Here we present a transcriptome-wide m(6)A modification profiling effort for rice transcriptomes of differentiated callus and leaf, which yields 8,138 and 14,253...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

The low yield and high market price of the pharmaceutically important alkaloids of Catharanthus roseus (L.) G. Don viz. vincristine, vinblastine and ajmalicine have created interest in improved alternative routes for their production such as using cell and tissue culture. The callus developed on Murashige and Skoog (MS) media supplemented with different concentrations of auxins and cytokinins w...

The ability of N,N'-diphenylurea (Ph(2)urea) to substitute for cytokinin-active adenine derivatives in promoting callus growth of Phaseolus lunatus has been examined. In general, Ph(2)urea stimulated callus growth at high concentrations, although the growth of most callus tissues was irregular. Variability in the sensitivity and uniformity of the growth response to Ph(2)urea was found among dif...