نتایج جستجو برای: callus mediated shoot formation

تعداد نتایج: 921532  

2010
Priti Maheshwari Anil Kumar

Plantlets were regenerated through callus-mediated organogenesis after UV–B irradiation (280–320 nm, 0.863–5.28 kJ m) in Vernonia cinerea and were analyzed by Random Amplified Polymorphic DNA with 10 random decamers to assess UV–B induced polymorphism. A total of 45 different somaclones were selected based on difference in morphology compared to the parent plant. Out of the 10 primers chosen fo...

Journal: :Bangladesh Journal of Botany 2022

Rice (Oryza sativa L.) is the world’s most important cereal crop. The Doairgura variety (O. a low-yielding, high-quality aromatic rice-producing in Bangladesh. This study was undertaken to develop an vitro regeneration protocol through callus induction. MS media supplemented with different concentrations of 2, 4- D (0.5, 1.0, 2.0 and 3.0 mg /l) were used for induction from mature dehusked rice ...

Journal: :Asian journal of agricultural and horticultural research 2022

The application of naphthalene acetic acid (NAA) different concentrations (25, 50, and 75 ppm) was applied to grafted mango seedlings identify its effect on callus formation, diameter, shoot emergence, plant survival. NAA effectively reduced the number days mainly using ppm. Lower than this concentration exhibited a comparable same as untreated seedlings. survival showed no significant by apply...

   Present study has comparatively investigated the effect of zinc oxide nanoparticles (ZnO NPs), ZnO bulk particles (BPs), and relevant metal ions (Zn2+) on the tissue culture of rapeseed (Brassica napus L.) to properly evaluate the impact of ZnO NPs on callus induction. For finding the best hormonal combination for inducing callus, hypocotyl explants were cultured on Murashige ...

Journal: :Plant physiology 1985
L D Beaudoin-Eagan T A Thorpe

Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased durin...

Journal: :Indian journal of experimental biology 2008
F Muniran Subhash J Bhore Farida H Shah

Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined...

Journal: :Journal of plant physiology 2003
Rakhi Chaturvedi Maharaj K Razdan Sant S Bhojwani

Triploid plants of neem were obtained by immature endosperm culture. Immature seeds, at the early dicotyledonous stage of embryo development, is the best explant to raise endosperm callus on MS + NAA (5 mumol/L) + BAP (2 mumol/L) + CH (500 mg L-1). Maximum shoot bud differentiation from the endosperm callus occurred on MS + 5 mumol/L BAP. Shoots were multiplied by forced axillary branching and ...

2016
Ayumi Amemiya Hiroshi Noguchi Makoto Oe Kimie Takehara Yumiko Ohashi Ryo Suzuki Toshimasa Yamauchi Takashi Kadowaki Hiromi Sanada Taketoshi Mori

Aim. Callus is a risk factor, leading to severe diabetic foot ulcer; thus, prevention of callus formation is important. However, normal stress (pressure) and shear stress associated with callus have not been clarified. Additionally, as new valuables, a shear stress-normal stress (pressure) ratio (SPR) was examined. The purpose was to clarify the external force associated with callus formation i...

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