Degrading cells of wheat and barley callus have all known ultrastructural features, which are characteristic for apoptotic cells: intense vacuolization, increasing the size of the nucleus, cupped invaginations of plastids, mitochondria saving until the last stages of cell degradation and the formation of vesicles, similar to apoptotic bodies. It is the first showed that degrading cells in embry...

Distraction osteogenesis is a biologic process of new bone formation between the surfaces of bone segments that are gradually separated by incremental traction. The process is initiated when distraction forces place tension on the callus between the bone segments, and continues as long as these tissues are stretched. This tension stimulates new bone formation parallel to the vector of distraction.

We have developed an intramedullary delivery strategy to administer retroviral vectors expressing a therapeutic gene to promote healing of a closed rat femur fracture. This strategy involves implantation of an indwelling catheter with the stabilizing Kirschner (K)-wire during the surgery prior to fracture of the femur by the three-point bending technique. It uses the openings in the bone that w...

PURPOSE To investigate the effect of extracorporeal shock wave treatment (ESWT) on bony union using volume analysis of the callus mass by computed tomography and histology. METHODS Both radii of 13 rabbits were osteotomised and a defective bony nonunion created by placing a polyethylene pad between the osteotomy site for 40 days. Nonunion was confirmed by radiography using Lane-Sandhu criteri...

An efficient protocol was developed for the induction of callus, somatic embryogenesis and regeneration response of two sugarcane varieties: HSF-243 and HSF-245, in this study. Callus was induced on young leaf explants cultured on Murashige and Skoog’s medium supplemented with different concentration of 2, 4-D singly or in combination with BAP. The highest percentage of callus induction was obs...

Degrading cells of wheat and barley callus have all known ultrastructural features, which are characteristic for apoptotic cells: intense vacuolization, increasing the size of the nucleus, cupped invaginations of plastids, mitochondria saving until the last stages of cell degradation and the formation of vesicles, similar to apoptotic bodies. It is the first showed that degrading cells in embry...

INTRODUCTION: Callus distraction is a well-accepted method for treating large bone defects. Unfortunately, the time for the maturation of a newly formed regenerate is long and can lead to complications. A method which would allow the acceleration of the maturation process would shorten the treatment time, reduce the costs and would be a great benefit for the patients. Plasma factor XIII has dem...

The synthetic growth regulant DPX 1840 (3,3a-dihydro-2-(p-methoxyphenyl)-8H-pyrazolo[5, 1-a]isoindol-8-one) induced callus growth and subsequent tissue differentiation on cut surfaces of decapitated Xanthi-nc tobacco plants (Nicotiana tabacum). Callus formation and organogenesis induced by DPX 1840 depended on the presence of leaves. The adventitious meristems developed into either vegetative o...