The first and main part of this thesis is focused on the design and synthesis of photo-activable and metal chelating alkanethiols. Chelate associated photochemistry (CAP) is a novel concept of combining two well-known protein (ligand) immobilization strategies to obtain a sensor surface of covalently bound ligand with defined orientation. This includes nitrilotriacetic acid (NTA) which is used ...

Glyoxal (CHOCHO) is an atmospheric trace gas produced by the radical-initiated oxidation of hydrocarbons. High resolution measurements of the glyoxal absorption spectrum in the visible wavelength range are needed as reference spectra to calibrate optical spectroscopic measurements of this gas. The UV-vis absorption spectra of C13-disubstituted glyoxal and ambient (C12) glyoxal were collected at...

INTRODUCTION In the opportunistic pathogen Pseudomonas aeruginosa, the production of several virulence factors depends on quorum sensing (QS) involving N-acylhomoserine lactone signal molecules. In vitro studies have suggested that the QS system is crucial in the pathogenesis of P. aeruginosa. However, it is unclear whether QS systems of P. aeruginosa play the same role during infections. MET...

We investigate the use of laccase enzymes to couple short nonpolar chains containing aromatic groups onto flax fibers and nanofibrillated cellulose (NFC) with different lignin contents. Trametes villosa , Pycnoporus cinnabarinus , and Myceliophthora thermophila were used to facilitate surface coupling and to produce materials with different levels of hydrophobicity. Heat treatment of fiber webs...

Abstract This study aimed to examine the ultrasonographic characteristics of mammary gland in post-partum ewes (1 4 weeks after lambing) varying genotypes for correlations with chemical composition milk. Ultrasonograms glands coronal (C) and sagittal (S) planes were obtained using 5.0- 7.5-MHz transducers from four Polish Mountain (PMt) six Olkuska (Olk) ewes. Computerized image analyses all im...

We measured the elastic constants (C11 − C12)/2 and C44 of non-Kramers system Y0.63Pr0.37Ir2Zn20 (Pr-37% system) by means ultrasound to check how single-site quadrupolar Kondo effect is modified increasing Pr concentration. The Curie-like softening present Pr-37% on cooling from 5 1 K can be reproduced a multipolar susceptibility calculation based Γ3 doublet crystalline-electric-field ground st...

Acinetobacter oleivorans DR1 can utilize C12 -C30 alkanes as a sole carbon source but not short-chain alkanes (C6 , C10 ). Two copies of each alkB-, almA- and ladA-type alkane hydroxylase (AH) are present in the genome of DR1 cells. Expression and mutational analyses of AHs showed that alkB1 and alkB2 are the major AH-encoding genes under C12 -C30 , and the roles of other almA- and ladA genes a...

Several novel phorbol esters were prepared with polar functional groups terminating their C12 and/or C13 acyl chains. Designed to be inhibitory protein kinase C (PKC) ligands, these phorbol analogues contain various polar functional groups (amide, ester, carboxylic acid, or quaternary ammonium salt) to prevent membrane insertion of the PKC-phorbol ester complex. All phorbol derivatives were syn...

We have examined intracellular transport and metabolism of the fluorescent analogue of phosphatidylserine, 1-palmitoyl-2-(N-[12[(7-nitrobenz-2-oxa-1,3-diazole-4-yl)amino] dodecanoyl])-phosphatidylserine ([palmitoyl-C12-NBD]-PS) in cultured fibroblasts. When monolayer cultures were incubated with liposomes containing (palmitoyl-C12-NBD)-PS at 37 degrees C, fluorescent PS was transported to the G...

Oncostatin M (OSM) is a member of a family of cytokines that includes ciliary neurotrophic factor, interleukin-6, interleukin-11, cardiotrophin-1, and leukemia inhibitory factor (LIF). The receptors for these cytokines consist of a common signaling subunit, gp130, to which other subunits are added to modify ligand specificity. We report here the isolation and characterization of a cDNA encoding...