نتایج جستجو برای: by extension

تعداد نتایج: 7109068  

Journal: :Annals of the New York Academy of Sciences 2015

Journal: :American Journal of Pharmacology and Toxicology 2008

Journal: :Proceedings of the National Academy of Sciences 2017

2012
Sanjeev Kumar Sophie D. Lefevre Marten Veenhuis Ida J. van der Klei

BACKGROUND Chronological aging of yeast cells is commonly used as a model for aging of human post-mitotic cells. The yeast Saccharomyces cerevisiae grown on glucose in the presence of ammonium sulphate is mainly used in yeast aging research. We have analyzed chronological aging of the yeast Hansenula polymorpha grown at conditions that require primary peroxisome metabolism for growth. METHODO...

Journal: :CSH protocols 2006
Joseph Sambrook David W Russell

1. Phosphorylate oligonucleotide probe: a. Oligonucleotide primer (5 – 7 pmoles or 60 ng) 1 μL b. dH2O 6.5 μL c. 10X kinase buffer 1.5 μL d. T4 PNK (~10 U) 1 μL e. [γ-P]ATP (7000 Ci/mmole) 2 μL 2. Stop the kinase reaction by adding 500 μL of TE (pH 7.6) 3. Add 25 μg of carrier RNA. 4. Phenol/Chloroform extract 2X 5. Ethanol precipitate with sodium acetate (pH 5.2) 6. Wash with 70% ethanol and r...

Journal: :Proceedings of the National Academy of Sciences 2002

Journal: :Illinois Journal of Mathematics 2008

Journal: :Proceedings of the National Academy of Sciences 1968

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