The purpose of our experiment was to investigate, if apparently healthy, vaccinated chickens may be involved in maintaining and spreading infectious bursal disease virus (IBDV) in poultry environments. We aimed at simultaneous detection and identification of very virulent field strain IBDV (vvIBDV) as well as vaccine strain IBDV in experimentally infected chickens. Two groups of specific pathog...

The objective of this study was to identify the quantitative trait loci (QTL) affecting carcass traits on chromosome 1 in Japanese quail. The populations comprised of 422 progeny in 9 half-sib families. Phenotypic data on carcass weight, carcass parts, and the internal organs were collected on 422 progeny. Nine half-sib families were genotyped for 8 microsatellite markers covering chromosomes 1...

The bursa of Fabricius (BF), which is unique to birds, serves as the central humoral immune organ and plays a significant role in B lymphocyte differentiation. In this study, a new bursal peptide (BP-IV) was isolated from BF, which promoted colony-forming unit pre-B formation and regulated B cell differentiation. BP-IV also exerted immunomodulatory effects on antigen-specific immune responses v...

A study of lymphoid-glandular complexes of the large bowel has been undertaken. Sections from 1924 surgical colectomy and proctocolectomy specimens were examined, and lymphoid-glandular complexes were observed in 231. It has been shown that they are distributed throughout the large bowel and occur in all age groups and in normal and disease states. An analogy has been drawn between them, the pa...

Molecules expressed on lymphoid stromal cells influence the differentiation of lymphocytes. We have examined the expression of stromal markers, identified by monoclonal antibodies, in the chicken bursa of Fabricius during ontogenic development. These results are also consistent with the hypothesis that medullary secretory cells are of mesenchymal origin, whereas the basement membrane-associated...

During embryonic development, the avian bursa of Fabricius selects B cell precursors that have undergone productive V(D)J recombination for expansion in oligoclonal follicles. During this expansion, Ig diversity is generated by gene conversion. We have used retroviral gene transfer in vivo to introduce surface Ig molecules that lack V(D)J-encoded determinants into B cell precursors. This trunca...

A model was developed to study stress in chickens. Continuous administration of adrenocorticotropic hormone was employed (ACTH) using physiological mini-osmotic pumps. A validation of controls for this procedure showed that nonhandled (NHCON), sham surgical procedure for pump implantation (SMCON) and surgical implantation of a pump delivering saline (SALCON) were all acceptable controls. Contin...

The Bursa of Fabricius of 15 day, 1-, 3-, and 6 month-old adult chickens (White Leghorn strain) were studied by histological and histochemical staining, histoenzymatic reactions (LDH, SDH, a-GPDH, NAD, NADPH, Ca++-dependent ATP-ase, pH 8.5) and by anti-thymostimulin immunoreaction. Positive reactions for mucopolysaccharides and enzymatic activities were located in the epithelia of the follicles...

To evaluate pathogenicity of 3 Chicken Anemia Virus isolates-CV1, CV2 and CV3 respectively - the current experiment carried out. After tittering of the viruses, 30 one day old SPF chicks were grouped and intramuscularly inoculated with each isolate in a separate group. Two other groups as positive and control groups were inoculate with a live vaccine virus and normal saline respectively. During...

The present study was aimed to administering hyperimmunized avian salmonella-immune lymphokines (S-ILK) 200 one-day-old layer pullets improve the immunological response against Gumboro (IBDV) divided into four groups. On first day, following applied all groups: G1: intraperitoneal injection of 0.50 mL S-ILK followed by a 30-minute challenge with 0.1 IBDV (ELD50 103.2); G2: 0.5 S-NILK IBDV. G3: ...