Brucella abortus is an intracellular pathogen of monocytes, macrophages, dendritic cells, and placental trophoblasts. This bacterium causes a chronic disease in bovines and in humans. In these hosts, the bacterium also invades neutrophils; however, it fails to replicate and just resists the killing action of these leukocytes without inducing significant activation or neutrophilia. Moreover, B. ...

Abstract Background The prevalence of human brucellosis in Qinghai Province China has been increasing rapidly, with confirmed cases distributed across 31 counties. However, the epidemiology transmission not fully elucidated. To characterize infecting strains isolated from humans, multiple-locus variable-number tandem repeats analysis (MLVA) and whole-genome single-nucleotide polymorphism (SNP)-...

Koser, Breslove, and Doirfman (1941) obtained moderate growth (one billion cells per ml) of the three species, Brucella melitensis, Brucella abortus, and Brucella suis, in a chemically defined medium consisting of 17 amino acids, glucose, inorganic salts, thiamin, nicotinamide, biotin, and pantothenic acid. All of the strains studied required thiamin and nicotinamide, and all weere stimulated b...

In a blind test, 344 samples representing 80 bacterial isolates were analyzed by the Brucella abortus species-specific polymerase chain reaction (BaSS PCR) assay for the identification and discrimination of B. abortus field strains (wild-type biovars 1, 2, and 4) from 1) B. abortus vaccine strains, 2) other Brucella species, and 3) non-Brucella bacteria. Identical samples were tested in 2 labor...

Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in mammals. Brucella strains containing the O-polysaccharide in their cell wall structure exhibit a smooth phenotype whereas the strains devoid of the polysaccharide show rough phenotype. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucell...

The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis, and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high ( > 99%) homology among the three species examined. Two genus-specific primer pairs, BRU-P5-BRU-P8 and BRU-P6-BRU-P7, that could be used in a nested PCR format and three genus-specific...

pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts’ defensive mechanisms. in this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their host’s killing attitude. studying the interface of host-pathogen confrontation can provide valuable information for defi...

PURPOSE Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative ti...

Brucellosis is an emerging zoonosis and an economically important infectious disease of livestock with worldwide distribution [14]. The disease is manifested by abortion and infertility and caused by Brucella spp. which are Gramnegative, facultative intracellular bacteria. Six species in the genus of Brucella are currently recognized on the basis of the phenotypic characteristics, antigenic pro...