The intracellular pathogen Brucella is the causative agent of brucellosis, a worldwide zoonosis that affects mammals, including humans. Essential to Brucella virulence is its ability to survive and replicate inside host macrophages, yet the underlying mechanisms and the nature of the replicative compartment remain unclear. Here we show in a model of Brucella abortus infection of murine bone mar...

Brucellosis is a zoonotic disease; its symptoms and appearances are not exclusive in human and its traditional diagnosis is based on culture, serological methods and conventional PCR. For more sensitive, specific detection and differentiation of Brucella spp., the real time PCR method is recommended. This research has performed to determine the presence and prevalence of Brucella spp. and diffe...

Brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. Despite animal vaccination, brucellosis remains endemic in some areas such as Iran, especially in the western Iranian province of Hamadan. We sought to detect some of the most common virulence-associated genes in Brucella isolated from human blood cultures to determine the prevalence of some v...

Brucella spp. were isolated from marine mammals for the first time in 1994. Two novel species were later included in the genus; Brucella ceti and Brucella pinnipedialis, with cetaceans and seals as their preferred hosts, respectively. Brucella spp. have since been isolated from a variety of marine mammals. Pathological changes, including lesions of the reproductive organs and associated abortio...

Neurobrucellosis has been reported to cause lesions in a number of different locations in the central nervous system. Histologically or radiologically, these lesions were consistent with an infection. In response to parents who believed their child's brain tumor, histologically typical of medulloblastoma, was in reality neurobrucellosis, formalin-fixed paraffin-embedded tumor tissue from the me...

Background: The intrinsic heterogeneity determination in Brucella Lipopolysaccharide (LPS) is important for explaining its chemicalnature and biological behavior. This is significant for practical purposes, since LPS is the most relevant antigen during infectionand vaccination.Objectives: The purpose of the present study was to compare biochemical and immunological differences of LPS and lipid ...

Brucellosis is a worldwide zoonosis that affects livestock and humans and is caused by closely related Brucella spp., which are adapted to intracellular life within cells of a large variety of mammals. Brucella can be considered a furtive pathogen that infects professional and non-professional phagocytes. In these cells Brucella survives in a replicative niche, which is characterized for having...

Brucellosis is a chronic granulomatous infection caused by intracellular Gram-negative bacteria belonging to Brucella species. While uncommon in the United States, northern Europe and Eastern Asia, it is common in the Middle East, southern Europe, and South America [1]. Human brucellosis is a potentially life-threatening multisystem disease which requires combined and prolonged treatment with a...

The objective of this study was to determine seroprevalence of small ruminant lentivirus (SRLV), Brucella abortus and Brucella melitensis infections among dairy goats in South Backa and Srem region in Vojvodina Province. The other aim was to determine the risk factors associated with SRLV infection in investigated herds. A total of 971 serum samples were collected from 8 comercial dairy goat he...

Three therapeutic regimens were evaluated in 121 cows naturally infected with Brucella melitensis or Brucella abortus, using a combination of long-acting oxytetracycline (LA-OTC), streptomycin (ST) and OTC-intramammary infusion (IMI). Cessation of shedding of Brucella in udder secretions and absence of Brucella in selected tissues were considered criteria for successful treatment. Regimen A (te...