CLINICAL CHEMISTRY. Vol. 26, No. 1, 1980 177 may then be used to make the precipitin lines visible. The stain that seemed to give the best contrast between precipitin lines and membrane background was Procion Brilliant Blue M-RS (Inolex Corp., Glenwood, IL). An aqueous stain stock solution, 1.2 g/L was prepared and membranes placed in a working mixture of stain stock/water/acetic acid/methanol ...

The potencies of mammalian cell-derived recombinant human erythropoietin pharmaceutical preparations, from a total of five manufacturers, were assessed by in vivo bioassay using standardized protocols. Eight-week-old normocythemic mice received a single subcutaneous injection followed by blood sampling 96 h later or multiple daily injections with blood sampling 24 h after the last injection. Re...

Airflow-enhanced evaporation is investigated as a method for rapid chemical preconcentration on a thin porous substrate. The mechanism is described by combining 1D models of capillary rise, chromatography, and pervaporation concentration. It is shown that the effective length of the column can be shorter than its actual length, allowing concentrate to be held at a stagnation point and then rele...

The heterogeneous photo-Fenton degradation of coomasie brilliant blue R–250 under visible light has been investigated using copper modified iron oxide, which has been prepared by coprecipitation method and characterized by IR spectroscopy, scanning electron microscopy and X-ray diffraction. The rate of photocatalytic degradation of dye follows pseudo-first order kinetics. The effects of various...

PURPOSE To evaluate macular retinal ganglion cell-inner plexiform layer (GCIPL) thickness changes after Brilliant Blue G-assisted internal limiting membrane peeling for idiopathic macular hole repair using a high-resolution spectral-domain optical coherence tomography (SD-OCT). METHODS 32 eyes from 32 patients with idiopathic macular holes who underwent vitrectomy with internal limiting membr...

A strain LN07 with high laccase yield was identified as basidiomycete fungus Lepista nuda from which a white laccase without type I copper was purified and characterized. The laccase was a monomeric protein with a molecular mass of 56 kDa. Its N-terminal amino acid sequence was AIGPAADLHIVNKDISPDGF. Besides, eight inner peptide sequences were determined and lac4, lac5 and lac6 sequences were in...

This study investigates the optimal conditions for preparation of corn cob based activated carbon (CCAC) for the removal of Remazol Brilliant Blue R (RBBR) reactive dye from aqueous solution. The CCAC was prepared by using physiochemical activation method which consisted of sodium carbonate (Na2CO3) and CO2 gasification. Central composite design (CCD) was used to determine the effects of the th...

A procedure permitting the isolation of large amounts of cytochrome b-559 from chloroplasts of barley is described. The isolated cytochrome b-559 has an apparent molecular weight of 120,000. The protein equivalent weight per mole of heme is 30,000. Depending on the electrophoretic conditions used, this molecule can be dissociated into components with apparent molecular weights of 31,000, 30,000...

Membrane fractions have been isolated from eggs of Fucus serrratus which inhibit fertilization in a species-specific manner. This activity is destroyed by alpha-fucosidase and alpha-mannosidase. Some 6% of the protein of this membrane fraction binds to Con A-agarose, following SDS solubilization, and when eluted with alpha-methyl mannoside inhibits fertilization when preincubated with sperm, bu...