Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resistance- and susceptibility-associated alleles of bovine NRAMP1 as stable transg...

During an investigation of bovine brucellosis in Iran, conducted by the Razi Institute over a twelve-month period, samples of serum and milk were collected simultaneously from 6,472 cows in eight infected herds for serological and bacteriological testing. A total of 1,056 cows were serologically positive and 1,632 of 6,472 milk samples were positive to the milk ring test (MRT). Culture of the p...

HERR, S., TE BRUGGE, LESLEY A. & GUINEY, M. C. M., 1982. The value of the microtitre serum agglutination test as a second screening test in bovine brucellosis. Oruierstepoort Journal of Veterinary Research, 49, 23--28 (1982). The use of the serum agglutination test (SAT) as a 2nd screening test led to a reduction in false negative screening reactions especially in sera delayed in transit and pr...

A macroscopic agglutination test for detecting leptospiral antibodies in human sera is described. The test utilizes a stained preparation of non-pathogenic leptospires and is performed on the Brewer diagnostic cards used for bovine brucellosis screening. The agglutination of the stained antigen is more easily observed than the current macroscopic slide test using unstained leptospiral antigens....

Serologic responses in the particle concentration fluorescence immunoassay and the card, complement fixation, and tube agglutination tests were measured for 10 weeks after vaccination of cattle with either Brucella abortus 19 or the lipopolysaccharide O-antigen-deficient mutant, strain RB51. The responses of strain 19-vaccinated cattle were positive, whereas those of strain RB51-vaccinated catt...

Numerous diseases of importance in domestic ruminants and free-ranging wildlife can be transmitted between each other. This section will be limited to diseases that are known to affect both wild and domestic animals. In most cases, the disease can be transmitted in both directions and have important impacts in both domestic and free-ranging animals. In some cases the disease is primarily in dom...

COMPARISON OF ENZYME IMMUNOASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS. The indirect enzyme immunoassay for measurement of bovine antibody to Brucella abortus was tested on 15,716 Canadian sera to assess the specificity. These sera were also tested by the buffered plate antigen test. Two ELISA formats were used for assessment of data: the targeting procedure using a positive control serum al...

We investigated the seroprevalence and risk factors for Brucella seropositivity in cattle in Jordan. The sera from 671 cows were randomly collected from 62 herds. The antibodies against Brucella were detected using a Rose Bengal plate test and indirect ELISA. A structured questionnaire was used to collect information on the cattle herds' health and management. A multiple logistic regression mod...

Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO– Farah, Mathura (UP); Department of Veterinary Microbiology and Immunology, Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Ewam Go–Anusandhan Sansthan (DUVASU), Mathura (U.P.)–281001; Division of Pathology, Indian Veterinary Research Institute, Izatnagar (IVRI), Bareilly (U.P...