A new form of ileostomy bag, attached to the skin by a latex fixative, is described. The need for a careful selection of cases and a gradual approach to the subject of ileostomy is emphasised. The modern operation of double-ended ileostomy is described, together with some improvements in technique. Treatment in the preoperative and postoperative stages is outlined ; patients must be thoroughly ...

Neutral buffered Formalin, a fixative used in most pathology laboratories, was found to inactivate human T-lymphotropic virus type III/lymphadenopathy-associated virus. Preparations containing this virus with infectivity titers of greater than 10(5) were treated with 1% or greater neutral buffered Formalin; after treatment, virus was undetectable (titer, less than 10(1)). In addition, when infe...

A characteristic type of reticular cell, the dense reticular cell is described and illustrated by electron microscopy in the thy mus and lymph nodes of mice. This cell is identified by its high nuclear and cytoplasmic density and by the presence of many ribosomes in its long, slender processes extending between lym phocytes (or thymocytes). The preservation of these processes requires special f...

mer. They inflated the resected lungs used in their study with 10% buffered formalin at a transpulmonary pressure of 25 cm H2O for a minimum of 24 hours. What technique did they use and how well were the lungs inflated? Satisfactory inflation via the bronchus of surgically resected lobes has been found unsatisfactory in two Vancouver hospitals. In one, expansion had to be achieved by inflation ...

A quantitative morphometric study of the immunoglobulin-containing plasma cells in rectal biopsies from nine patients with ulcerative colitis and three patients with the irritable colon syndrome is presented. The results show that fixation of the biopsy specimens with formol-sublimate, a mercuric fixative, resulted in better staining with the immunoperoxidase method and that, by comparison with...

This article presents an improvement to the Kato-Katz (KK) method, making it faster and more efficient for the visualisation of fertile eggs in stool samples. This modified KK method uses sodium acetate formalin as a fixative and reveals the intensity of infection in less than 1 h, reducing the diagnostic time without increasing the cost. This modified method may contribute to future epidemiolo...

INTRODUCTION Electron microscope studies of yeasts have revealed the laminated structure of the cell wall (1, 9, 10, 19), its major chemical constituents being distributed in separate layers (13, 15) . The number of layers and their morphology are, however, a matter of controversy mainly because the methods of chemical fixation of yeast cells for electron microscopy employed to date have been i...

Members of the genus Habronema (Nematoda: Habronematidae) were preserved in 4 fixatives for examination with scanning electron microscopy (SEM). Tegumental features of the anterior and posterior extremities of these specimens were compared to evaluate the effect of the fixatives: modified Flemming's solution, glutaraldehyde (GA), Karnovsky's fixative and 70% ethanol. Fixatives were assessed on ...

For chromosomal analysis in tumor genetics, cells from blood and bone marrow are prepared and preserved virtually indefinitely in Carnoy's fixative (methanol/acetic acid). Numerous samples are stored unvalued in hospitals and institutes worldwide. We developed a method to analyze proteins from even a small amount of these cells by mass spectrometry using affinity chromatographic surfaces (SELDI...