Phosphoinositide-3-kinase and protein kinase B (PI3K-AKT) is upregulated in multiple myeloma (MM). Using a combination of short hairpin RNA (shRNA) lentivirus-mediated knockdown and pharmacologic isoform-specific inhibition we investigated the role of the PI3K p110γ (PI3Kγ) subunit in regulating MM proliferation and bone marrow microenvironment-induced MM interactions. We compared this with inh...

INTRODUCTION The occurrence of skeletal metastases in cancer, e.g. breast cancer (BC), deteriorates patient life expectancy and quality-of-life. Current treatment options against tumor-associated bone disease are limited to anti-resorptive therapies and aimed towards palliation. There remains a lack of therapeutic approaches, which reverse or even prevent the development of bone metastases. Rec...

This retrospective report presents findings on 7 patients with idiopathic dilated cardiomyopathy who underwent transplantation of autologous bone marrow derived mononuclear cells (BMSC) after failed routine conservative treatment, and were on a waiting list for cardiac transplantation. In two out of seven patients, we performed intramyocardial delivery of BMSC twice, 22 and 24 months respective...

Background: Spinal cord injury (SCI) is a serious traumatic disease of the central nervous system, and there currently no effective treatment for SCI because its complicated pathophysiology. Bone marrow mesenchymal stem cells (BMSCs) have multidirectional differentiation abilities. Our study aims to explore effects bone morphogenetic protein 7 (BMP-7)-modified BMSCs transplantation on repair in...

BACKGROUND AIMS Transplantation of mesenchymal stromal cells (MSC) derived from bone marrow (BM) or adipose tissue is expected to become a cell therapy for stroke. The present study compared the therapeutic potential of adipose-derived stem cells (ASC) with that of BM-derived stem cells (BMSC) in a murine stroke model. METHODS ASC and BMSC were isolated from age-matched C57BL/6J mice. These M...

For tissue engineering of bone, a carrier matrix and efficient cell seeding are desirable. This study analysed the effect of fibrin glue on bone marrow stromal cells (BMSC) adhesion, proliferation (MTS-Test), differentiation (alkaline phosphatase (AP), osteocalcin (OC), ELISA) and compared the results with cells seeded within culture media on a decellularized, xenogenic bone matrix. There was n...

objective: tendon never returns to its complete biological and mechanical properties after repair. bone marrow and, recently, adipose tissue have been used as sources of mesenchymal stem cells which have been proven to enhance tendon healing. in the present study, we compared the effects of allotransplantation of bone marrow derived mesenchymal stromal cells (bmscs) and adipose derived stromal ...

Selection of appropriate osteoinductive growth factors, suitable delivery method and proper supportive scaffold are critical for a successful outcome in bone tissue engineering using bone marrow stromal cells (BMSC). This study examined the molecular and functional effect of a combination of adenoviral mediated expression of bone morphogenetic protein-2 (BMP2) in BMSC and recently developed and...

In this article, the author reviews recent advancements of basic research on bone marrow stromal cell (BMSC) transplantation for ischemic stroke. The BMSCs are easily isolated from the patients themselves and transplanted into them without any ethical and immunological problem. Animal experiments have shown that BMSC transplantation significantly enhance the recovery of motor and/or cognitive f...