The quick blot protocol is an improved technique for preparing crude insect homogenates for hybridization to nucleic acid probes. Individual insects are ground in wells of a microtiter plate and transferred to a dot blot manifold. This allows preparation of multiple filters and provides uniformity and an orderly arrangement of samples. The high background detection resulting from use of crude i...

1.Liu, Z., D.B. Batt and G.G. Carmichael. 1994. Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA. Proc. Natl. Acad. Sci. USA 91:4258-4262. 2.Sawadogo, M. and R.G. Roeder. 1985. Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. Proc. Natl. Acad. Sci. USA 82:4394-4398. 3...

This work aimed to evaluate the effect of Semaphorin 4D (SEMA4D) signaling through Plexin B1 on vasculogenic differentiation dental pulp stem cells. We assessed protein expression SEMA4D and in cells (DPSC) from permanent human teeth exfoliated deciduous (SHED) using Western blots. Their tissues DPSC-engineered pulps was determined immunofluorescence. then exposed recombinant (rhSEMA4D), evalua...

Previous studies have reported the up-regulated expression of protein O-fucosyltransferase 1 (POFUT1) in several human malignant tumors. This study purposes to explore relevance POFUT1 clinical and biological characteristics salivary gland adenoid cystic carcinoma (SACC). 54 SACC samples were applied immunohistochemistry evaluate practical POFUT1. The cells from submandibular (HSG), cell lines ...

β-Sheet-rich aggregates of α-synuclein (αSyn) are the hallmark neuropathology of Parkinson's disease and related synucleinopathies, whereas the principal native structure of αSyn in healthy cells--unfolded monomer or α-helically folded oligomer--is under debate. Our recent crosslinking analysis of αSyn in intact cells showed that a large portion of endogenous αSyn can be trapped as oligomers, m...

Phytoplasmas, uncultivable phloem-limited phytopathogenic wall-less bacteria, represent a major threat to agriculture worldwide. They are transmitted in a persistent, propagative manner by phloem-sucking Hemipteran insects. Phytoplasma membrane proteins are in direct contact with hosts and are presumably involved in determining vector specificity. Such a role has been proposed for phytoplasma t...

This article has been retracted at the request of Microbiology because identical bands for the 16S rRNA probe controls in the Northern blots were reported to correspond to experiments using different strains and experimental conditions in articles published in this journal and in Journal of Bacteriology over a period of 5 years.

What’s in your product? Come hear the latest advances in the field of biopharmaceutical HCP Testing ● Rare Reagents: Multiple Case Studies on how to develop your rare reagents, including selection of null cell lines, developing immunization strategies and assessing the specificity of your antibodies, characterization of antibodies and standards by 2-D Gels, western blots, and immunofractionation

The original publication of the article listed above did not clearly state that the representative blots in Fig. 6 are composed of four separate images. As such, the reader may mistakenly presume that the representative bands in Fig. 6 were taken from contiguous lanes of a single blot as in Fig. 4. The purpose of this corrigendum is, first, to clarify that the representative bands in the inset ...