BACKGROUND Bleomycin hydrolase (BLMH), an enzyme that inactivates bleomycin, may be a potential candidate that could influence pulmonary function in ABVD (doxorubicin, bleomycin, vinblastin, dacarbasine)-treated Hodgkin lymphoma (HL) patients. PATIENTS AND METHODS We hypothesized that the BLMH gene SNP A1450G (rs1050565) influences BLMH activity and late pulmonary toxicity. St. George Respira...

Epithelial cells are considered to be a main target of bleomycin-induced lung injury, which leads to fibrosis in vivo. We studied the characteristics of in vitro bleomycin-induced apoptosis in a mouse lung epithelial (MLE) cell line. Bleomycin caused an increase of reactive oxygen species (ROS) resulting in oxidative stress, mitochondrial leakage, and apoptosis. These were associated with eleva...

The mechanisms involved in bleomycin-induced lung toxicity have not been fully understood to date. This work aimed to compare the proteome profiling of bleomycin-induced lung toxicity by using 2D-nano-LC-MS/MS and spectral counting. By comparing the spectral counts of identified proteins between control and bleomycin-treated groups, we noted that 102 proteins were upregulated and 28 proteins we...

Hydrogel nanospheres composed of methacrylic acid and poly(ethylene glycol) were loaded with bleomycin and tested as a potential oral delivery system for chemotherapeutic agents. The gastrointestinal epithelium was modeled through the use of Caco-2 monolayers for studies of permeation enhancement by the carriers as well as bleomycin transport. Bleomycin efficacy following release from the carri...

Radiotherapy and chemotherapy are effective cancer treatments due to their ability to generate DNA damage. The major lethal lesion is the DNA double-strand break (DSB). Human cells predominantly repair DSBs by non-homologous end joining (NHEJ), which requires Ku70, Ku80, DNA-PKcs, DNA ligase IV and accessory proteins. Repair is initiated by the binding of the Ku heterodimer at the ends of the D...

BACKGROUND In metastatic testicular cancer patients treated with bleomycin, etoposide, and cisplatin (BEP) chemotherapy, bleomycin-induced pneumonitis is a well-known and potentially fatal side effect. We sought to determine the prevalence of lesions as signs of bleomycin-induced pulmonary changes on restaging computed tomography (CT) scans after treatment and to ascertain whether fibrosis mark...

Between December 1985 and August 1988, there were 115 patients at 13 centers who were entered on a randomized comparison of tetracycline and bleomycin for treatment of malignant pleural effusions. Fifteen patients were not treated, primarily due to rapid progression of systemic cancer. Fifteen patients entered on a high-dose regimen of bleomycin (120 units) were excluded from this analysis (fol...

The rejoining of DNA single-strand breaks produced after bleomycin treatment or gamma-irradiation of human diploid fibroblasts was studied by the alkaline elution technique. DNA rejoining occurred at slower rates in bleomycin-treated human fibroblasts than in gamma-irradiated fibroblasts. These comparisons were made at similar levels of survival or DNA single-strand breaks (including alkali-lab...

Background: Systemic sclerosis(SSc) is characterized by progressive fibrosis of the skin and internal organs. The change in epidermal–dermal interactions potentially causes abnormal differentiation SSc epidermis. However, blockage increased Ca2+ signaling restrains pathogenesis SSc-bleomycin mice. activation facilitates induction keratinocyte differentiation, which regulated stromal interaction...

Introduction . Bleomycin is a cytotoxic antibiotic that used as sclerosing agent for sclerotherapy of vascular anomalies. characterized by complications: nausea, vomiting, fever, the development allergies/anaphylaxis, skin discoloration (hyperpigmentation), damage to lung tissue. Aim To study frequency hyperpigmentation after administration bleomycin malformations with slow flow characteristics...