infectious bovine rhinotracheitis is a highly contagious disease caused by the bovine herpes virus-1 (bohv-1), resulting in significant losses to livestock around the world. bohv-1 is a major pathogen of cattle, primarily associated with respiratory/genital tract infections and abortion. in the present study, we determined the presence of antibodies in 120 serum samples of cattle with the histo...

introduction: monoclonal antibodies labeled mainly with 99mtc are being widely used as imaging agents in nuclear medicine. recently pr81 was introduced as a new murine anti-muc1 mab against human breast carcinoma. this antibody reacts with the tandem repeat of 20-mer peptide of protein backbone, and is of igg1 class, with an affinity of 2.19×10-8 m-1. due to high specific reactivity, this antib...

results isotype of 1f1 clone was determined to be igg1, which reacted with native protein in the western blot experiment and stained 20% of the membrane of neoplastic cells overexpressing her2 with 3+ grade. however, 3l5 clone showed a low reaction (10%) with native her2 in immunohistochemistry. conclusions the results of both western blotting and immunohistochemistry showed that native her2 ca...

introduction: measles is a severe contagious disease preventable by vaccination. besides the prevalence of measles in iran, ministry of health conducted a nation-wide vaccination against measles for all the 5-25 years old population in 2003. this study was done to evaluate the anti measles igg antibody among the medical students from baqiyatallah university of medical science. materials & metho...

background: during the last twenty years, the extraction of specific egg yolk (igy) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. the aim of the present study was immunizing the chickens with human umbilical cord serum (hucs) and the extraction of specific anti-human globulins (igg, c3b, and c3d) antibodies from egg yolk in orde...

background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

results based on our results, mab against recombinant erns was produced and reacted successfully with recombinant and natural antigens. conclusions with regards to the role of erns in the identification of pi animals, it appears that erns recombinant antigen and the specific monoclonal antibodies produced against it may be suitable for developing bvdv laboratory diagnostic assays. objectives er...

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

background: invariant natural killer cells (inkt) are an important immunoregulatory t cell subset. currently several flow cytometry-based approaches exist for the identifi-cation of inkt cells, which rely on using the 6b11 monoclonal antibody or a combina-tion of anti-vα24 and anti-vβ11 antibodies. objective: the aim of this study was to compare the ability of two flow cytometry-based methods f...

background: myocardial infarction (mi) is one of the most common and serious diseases resulting from coronary artery occlusion and major reduction in blood flow. streptokinase as a thrombolytic is considered the first and most important therapeutic intervention for reperfusion following mi in most countries including iran. our previous study showed that, the prevalence of high antibody titers a...