During the course of some studies on xanthme oxidase it was discovered that when the enzyme was incubated with one of its substrates in the presence of either lO , lO’-dimethyl-9,9’-biacridylium nitrate or 5-amino-2,3-dihydrophthalazine-l , 4-dione(luminol)light was produced in considerably greater intensity than would be expected from the rate of production of Hz02 and the known chemiluminesce...

Bioluminescence recording of Ca(2+) signals with the photoprotein aequorin does not require radiative energy input and can be measured with a low background and good temporal resolution. Shifting aequorin emission to longer wavelengths occurs naturally in the jellyfish Aequorea victoria by bioluminescence resonance energy transfer (BRET) to the green fluorescent protein (GFP). This process has ...

A multi-modal optical imaging system for quantitative 3D bioluminescence and functional diffuse imaging is presented, which has no moving parts and uses mirrors to provide multi-view tomographic data for image reconstruction. It is demonstrated that through the use of trans-illuminated spectral near infrared measurements and spectrally constrained tomographic reconstruction, recovered concentra...

ZnO nanorod was prepared by microwave assisted method. The crystal structure of the nano powders were confirmed by X-Ray diffraction analysis and the mean particle size was estimated by the Scherrer,s formula .The surface morphology of the nano particles were analyzed by   using SEM . The absorption spectrum of the material in the UV-Vis range was recorded .The energy band gap of the...

ZnO nanorod was prepared by microwave assisted method. The crystal structure of the nano powders were confirmed by X-Ray diffraction analysis and the mean particle size was estimated by the Scherrer,s formula .The surface morphology of the nano particles were analyzed by   using SEM . The absorption spectrum of the material in the UV-Vis range was recorded .The energy band gap of the...

BACKGROUND The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because bioluminescence itself may affect growth rate, e.g. by diverting energy. METHODOLOGY/PRINCIPAL FINDINGS The marker effec...

In conventional microscopes, fluorescence emission is separated from the backscattered illumination using the Stokes shift, whereby the emission occurs at a longer wavelength to the excitation. Such separation is usually achieved through a combination of wavelength filters that divide the spectrum into mutually exclusive excitation and emission bands. It is therefore impossible in these microsc...

Static properties, relatively intensity noise and intensity modulation response in quantum cascade lasers (QCLs) studied theoretically in this paper. The present rate equations model consists of three equations for the electrons density in the conduction band and one equation for photons density in cavity length. Two equations were derived to calculate the noise and modulation response. Calcula...