The utility of wild-type and variant carboxymethylproline synthases for biocatalysis was demonstrated by preparing functionalised 5-, 6- and 7-membered N-heterocycles from amino acid aldehydes and (alkylated) malonyl-coenzyme A derivatives; the N-heterocycles produced were converted to the corresponding bicyclic beta-lactams by a carbapenem synthetase.

Microwave irradiation can be used to regulate biocatalysis. Herein, the utilization of hyperthermophilic enzymes in a microwave reactor is reported. While these enzymes are inactive at low temperatures, they can be activated with microwave irradiation. To the best of our knowledge, this is the first illustration of a specific microwave effect in enzymatic catalysis.

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy probes the otherwise inaccessible structural information complex biological systems. We recently extended SDSL-EPR to reveal relative orientation and backbone dynamics of enzymes upon encapsulation mesoporous nanostructures, which set basis underlying observed biocatalytic activity. Our str...

The often reported reproducibility crisis in the biomedical sciences also applies to enzymology and biocatalysis, mainly results from incomplete reporting of reaction conditions. In this Concept article, an infrastructure based on EnzymeML is sketched, which enables reporting, exchange, storage enzymatic data according FAIR principles. a novel exchange format for facilitates application STRENDA...

• Water by far is the most popular solvent for biocatalytic transformations also because it widely considered as green water not ideal many hydrophobic reagents resulting in low reagent concentrations and large waste loads Non-aqueous reaction conditions offer opportunities to make biocatalysis more practical environmentally benign