To elucidate the IgE binding site of mugwort (Artemisia vulgaris r.) pollen, pollen grains were frozen and fixed using a cryocut. They were incubated with antibodies according to the following sequence: Sera pool of individuals who showed mugwort-RAST class 3 or 4, biotin-labeled goat anti-human IgE antibody, streptavidin-peroxidase and diaminobenzidine. Then, they were observed under electron ...

A simple bound is presented for the probability that the sum of nonnegative independent random variables is exceeded by its expectation by more than a positive number t. If the variables have the same expectation the bound is slightly weaker than the Bennett and Bernstein inequalities, otherwise it can be significantly stronger. The inequality extends to one-sidedly bounded martingale differenc...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

the binding of sodium n-dodecyl sulphate (sds) to histone h3 was studied in the ph range 3.2-10 by equilibrium dialysis at 27? and 3 7 ?c .t he binding data have been used to obtain the gibbs free energy of interaction using a theoretical model of the wyman binding potential; and the enthalpy of interaction from the temperature dependence of theequilibriumconstantsfronr thevan't hoff re1at...

the present study was an attempt to investigate varieties of cloze test and c-test as measures of reading comprehension ability. it aimed at comparing the performances of subjects on different cloze and c-test forms constructed from the same passage. to this end, five versions of cloze test and two versions of c-test were developed by applying different deletion techniques and deletion rates, a...

Computation of enabled transition instances is a key but difficult problem of animation of colored Petri nets. To address it in our colored Petri net tool, we give an algorithm for computing enabled transition instances. This algorithm is based on pattern matching. So it first tries to bind tokens to variables covered by patterns. If some variables are not covered by any pattern, the algorithm ...

Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...

There are questions concerning the functions of artemin, an abundant stress protein found in Artemiaduring embryo development. It has been reported that artemin binds RNA at high temperatures in vitro, suggesting an RNA protective role. In this study, we investigated the possibility of the presence of RNA-bindingsites and their structural properties in artemin, using docking energy ...