PURPOSE To examine ultrastructurally the composition of extracellular matrix (ECM) materials, the distribution of myocilin, and the colocalization of myocilin with ECM components in the juxtacanalicular tissue (JCT) of normal human eyes. METHODS Postembedding immunoelectron microscopic studies were performed with antibodies specific for major ECM components, including fibronectin, laminin, vi...

(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...

An electric field causes partial alignment of macromolecules in a dilute solution. The accompanying changes in the solution birefringence offer a sensitive and quick means of monitoring the rates of particle orientation and hence the size of the solute molecules. Such measurements are reported for dilute solutions of proteoglycans in the absence and presence of added hyaluronic acid. The proteo...

1. Dissociation of purified proteoglycan aggregates was shown to release an interacting component of buoyant density higher than that of the glycoprotein-link fraction of Hascall & Sajdera (1969). 2. This component, which produced an increase in hydrodynamic size of proteoglycans on gel chromatography, was isolated by ECTEOLA-cellulose ion-exchange chromatography and identified as hyaluronic ac...

Hyaluronic acid-based fillers are the most used dermal today. Due to its safety, several types of products on market with different rheological properties. Recently a new Korean filler high hyaluronic acid concentration was launched in Brazil. The properties this caught our attention and encouraged use for global facial rejuvenation. We report clinical experience these highly concentrated cohes...

Hyaluronic acid is the currently most used filler in dermatology due to its low risk of adverse events. The objective of this study is to report a case of granulomatous reaction after filling with two types of hyaluronic acid, in the perioral region and in the nasolabial folds. A female patient with rheumatoid arthritis treated with leflunomide presented onset of symptoms 30 months after fillin...

(1) The spreading reaction is a biological method, measuring the decomposing effect of hyaluronidase on the mucoids of the cement substance of rabbit's skin (Duran-Reynals, 1929). These substances consist partly of hyaluronic acid. Hyaluronidase increases the permeability of the tissues, and this involves an increased spreading capacity of liquids and corpuscular substances. This test is not sp...