The metabolism of [3H]benzo(a)pyrene ([3H]BP) in bovine aortic endothelial and bovine lung fibroblast-like cells in vitro was investigated. Both cell types metabolized BP to organic solvent-extractable and water-soluble metabolites. The major organic solvent-extractable metabolites were 9-hydroxy-benzo(a)pyrene and 3-hydroxybenzo(a)pyrene; 7,8-dihydro-7,8-dihydroxybenzo(a)pyrene, 9,10-dihydro-9...

Cytochrome P450 1A1 (CYP1A1) is a member of the cytochrome p450 enzyme family, which is involved in the metabolisms of carcinogenic metabolites, such as benzo(a) pyrene. In this study, we identified miR-892a as a negative regulator of CYP1A1 expression. Luciferase assays revealed a sequence in the 3'-untranslated region of CYP1A1 that displayed a perfect match with miR-892a, and revealed that t...

In order to compare the interactions of procarcinogens with mammary cells from humans and rats, a uniform set of mediated mutagenesis assays has been established. In these assays, species-specific mammary epithelial cells activate procarcinogens, and specific locus mutations are quantitated in cocultured V-79 cells. Mutations were induced in the rat mammary cell coculture system exposed to 7,12...

As test substances we selected naphthalene, benzo[a]pyrene and anthracene. Benzo[a]pyrene was selected, as the most common PAH required for the determination, as it is a component of most PAH mixtures and a procarcinogen, and is usually determined as an individual target PAH substance (http://water.epa.gov/drink/contaminants/basicinformation/benzo-a-pyrene.cfm). Naphthalene and anthracene are s...

A previous report from this laboratory has shown that certain derivatives of polycyclic aromatic hydrocarbons bind to phiX174 DNA and render it noninfectious. The present work describes the relationship between the extent of phiX174 DNA binding by (+/-)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide and the effect on infectivity. The results suggest that one molecule of bound diolepoxide is s...

A refined diet supplemented with Japanese-style fermented soy sauce (shoyu) inhibits benzo[a]pyrene-induced forestomach neoplasia in mice (Cancer Res., 51:2940-2942, 1991). In the present study, soy sauce was extracted with ethyl acetate. The soluble fraction contained flavor/aroma compounds and antioxidants, whereas amino-carbonyl compounds that impart color were concentrated in the ethyl acet...

The rote of prostaglandin endoperoxide synthetase in thà ̈ in vìvo activation of benzo(a)py rene to reactive metabolites capable of interacting irreversibly with cellular macromolecules was stud ied in guinea pig liver, lung, kidney, spleen, small intestine, colon, and brain. DNA and protein covalent binding experiments were made after systemic administration of acetylsalicylic acid (200 mg/kg)...

one of the problems in an hPlC laboratory is the preservation of samples and extracts prior to analysis without any changes. It has been found that degradation processes cannot be eliminated entirely, but they can be slowed down considerably. Photodegradation kinetics of fluorene, anthracene and benzo(a)pyrene were studied in various organic solvents. Solvents known as good media to store PAhs ...

Magnetic graphene-based composite nanomaterials were developed to capture benzo( a )pyrene from water media. The synthesized couple very easy magnetic separation after adsorption with highly efficient removal of ∼99.9%.