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OBJECTIVES To search for a new clinical application of melittin (Mel): treating hepatocellular carcinoma with Mel gene. METHODS Recombinant adenoviruses carrying the Mel gene and alpha-fetoprotein (AFP) promoter (Ad-rAFP-Mel) were constructed through a bacterial homologous recombinant system. The efficiency of adenovirus-mediated gene transfer and the inhibitory effect of Ad-rAFP-Mel on the p...

Calcium-independent phospholipase A(2)γ (iPLA(2)γ) (PNPLA8) is the predominant phospholipase activity in mammalian mitochondria. However, the chemical mechanisms that regulate its activity are unknown. Here, we utilize iPLA(2)γ gain of function and loss of function genetic models to demonstrate the robust activation of iPLA(2)γ in murine myocardial mitochondria by Ca(2+) or Mg(2+) ions. Calcium...

Material surface topography is an important factor for regulating cellular behaviour. Understanding the mechanism of how surface topography influences mammalian cells is critical for the development of medical implants and tissue engineering. In this study, we investigated the influences of nanoporous and microgrooved substrates on the morphology and migration of hepatic cell line, BEL-7402 cel...