Alginate beads represent a simple and highly reproducible in vitro model system for diffusion-limited bacterial growth. In this study, alginate beads were inoculated with Pseudomonas aeruginosa and followed for up to 72 h. Confocal microscopy revealed that P. aeruginosa formed dense clusters similar in size to in vivo aggregates observed ex vivo in cystic fibrosis lungs and chronic wounds. Bact...

Three strains of the freshwater microalgae used for wastewater treatment, Chlorella vulgaris and Chlorella sorokiniana co-immobilized separately in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense Cd, resulted in significant changes in microalgal-population size, cell size, cell cytology, pigment, lipid content, and the variety of fatty acids produced in com...

The phagocytic ability of amoebae of the cellular slime mold Polysphondylium pallidum, grown in shaken suspension, was examined. An established quantitative assay of the uptake of polystyrene (PS) beads was shown to be valid for this organism. The kinetics of phagocytosis were determined, and estimates of the concentration of PS beads necessary to achieve half-maximal phagocytic velocity (K(p))...

Abstract: Background: Inflammatory bowel disease (IBD) is a chronic inflammatory of large intestine consisting ulcerative colitis and Crohn’s disease. Mesalamine first line drug use for the treatment but it has drawback low water solubility permeability. In addition, oral delivery to colon faces problem gastric degradation thus increases dose size frequency. Objectives: The present study was ai...

Acrylic bone cements have been used for about 40 years to fix artificial prosthesis to bone structure. The properties of the acrylic bone cement mostly depend on the characteristics of the beads, which are the main component of the solid part of the cement. In this work beads of poly(methyl methacrylate-co-styrene) were synthesized by suspension polymerization. The objective of this study was t...

Electrowetting-on-dielectric (EWD) droplet actuation has been used widely in handling liquid in small quantities in many biological applications (Fair et al. 2007; Miller and Wheeler 2009; Boles et al. 2011; Welch et al. 2011). The droplet is manipulated by applying voltage to EWD electrodes buried beneath an insulator (Fair et al. 2007; Boles et al. 2011; Welch et al. 2011). Many droplet funct...

A method is described to measure the refractive index dispersion with wavelength of optically trapped solid particles in air. Knowledge of the refraction properties of solid particles is critical for the study of aerosol; both in the laboratory and in the atmosphere for climate studies. Single micron-sized polystyrene beads were optically trapped in air using a vertically aligned counter-propag...