We have grown the EHS (Engelbreth-Holm, Swarm) tumor in normal and genetically diabetic mice (db/db) and measured some components of basement membrane produced in the tumor. These studies showed similar amounts of total protein in control and diabetic tissue and similar patterns of proteins on SDS gel electrophoresis of extracts of the tissue. Laminin, a basement membrane specific glycoprotein ...

The Caenorhabditis elegans teneurin ortholog, ten-1, plays an important role in gonad and pharynx development. We found that lack of TEN-1 does not affect germline proliferation but leads to local basement membrane deficiency and early gonad disruption. Teneurin is expressed in the somatic precursor cells of the gonad that appear to be crucial for gonad epithelialization and basement membrane i...

The distributions of defined basement membrane proteins in nine pure tubular carcinomas, 10 cases of sclerosing adenosis, and 15 ductal adenocarcinomas were compared. Sections of formalin fixed, paraffin embedded specimens were pretreated with pepsin and then immunostained for laminin, type IV collagen, and basement membrane proteoglycan, components specific for basement membranes. In sclerosin...

Nephritis in rats was induced by intravenous injection of purified ferritin-conjugated rabbit and duck nephrotoxic globulin. Using the fluorescent antibody technique, the same capillary pattern was found as that in glomeruli of rats receiving uncoupled nephrotoxic globulin. Electron microscopy revealed a heavy accumulation of the basement membrane-fixed antibody almost exclusively at the endoth...

BACKGROUND Goodpasture's syndrome is a triad of alveolar hemorrhage, Glomerulonephritis and circulating anti Glomerular basement membrane antibodies, 25% of cases test positive for ANCA antibodies, this association is known as Double positive disease. This article describes a rare presentation of this entity and reviews available literature. CASE REPORT 80 year old female presented with hemop...

Insights into the role that inflammatory mediators such as lymphocytes and matrix metalloproteinases play in remodeling of the basement membranes of salivary gland cells in patients with Sjögren’s syndrome has led to several observations revealing irregular thickening and thinning of the basement membrane. These observations have revealed that the underlying mechanism for these irregularities i...

The mechanism underlying immune system recognition of different types of pathogens has been extensively studied over the past few decades; however, the mechanism by which healthy self-tissue evades an attack by its own immune system is less well-understood. Here, we established an autoimmune model of melanotic mass formation in Drosophila by genetically disrupting the basement membrane. We foun...

A new type of extracellular fibril is described in the dermis of Bufo marinus, Rana pipiens, and Amblystoma punctatum. It is restricted in distribution to the dermal micropapillae and the region immediately below them in the stratum spongiosum. The fibrils (diameter = 200 to 750 A) are lateral aggregates of fine filaments and have a unique banding pattern characterized by absence of recognizabl...

Tunicamycin (TM), an antibiotic that selectively inhibits dolichol-mediated protein glycosylation, inhibited morphogenesis and differentiation of odontoblasts in the molar tooth germ in vitro. These effects of TM are reversible and dose-dependent, and in advanced teeth the effect of TM was not complete unless the basement membrane was removed prior to culture. TM did not prevent secretion of pr...

A case of mixed membranous and proliferative glomerulonephritis has been studied by light and electron microscopy. Electron microscopy has shown that the cellular proliferation is mesangial in origin and that there are qualitative and quantitative alterations in the filtration basement membrane. In addition there are numerous examples of direct contact between filtration basement membrane and n...